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Table 1 General statistics for the normalized libraries.

From: Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis

 

MS

RE

REH

FRE

# Reads*

11956

10659

10441

10340

# average read length (bp)

604

585

606

600

# singletons

4863

3881

4480

4205

# clusters

2380

1804

2014

2032

# putative transcripts

7243

5687

6494

6237

Avg cluster size

2.98

3.76

2.96

3.02

Largest cluster

13

31

19

18

# clusters with 2 ESTs

1251

854

1120

1106

# clusters with 3 ESTs

547

383

449

416

# clusters with 4–5 ESTs

428

294

304

349

# clusters with 6–10 ESTs

146

192

129

156

# clusters with > 10 ESTs

8

81

12

5

Gene discovery

0.61

0.53

0.62

0.60

Gene diversity

0.41

0.36

0.43

0.41

# (%) with significant* SwissProt hits**

3846 (53%)

2947 (52%)

3110 (48%)

3402 (55%)

# (%) with significant* trembl hits**

4172 (58%)

3134 (55%)

3353 (52%)

3645 (58%)

# (%) with no hits

3030 (42%)

2517 (44%)

3107 (48%)

2555 (41%)

  1. * Reads that are submittable and > 100 bp. ** Threshold for Blastx significance = 1e-10. Gene discovery is defined as the number of different "genes" each library contributed, divided by library size. Gene Diversity is defined as the number of singletons in each library divided by library size [22].