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Table 2 General statistics for the subtractive libraries.

From: Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis

 

sbs01

sbs02

sbs03

sbs04

# Reads*

1021

1189

1117

1203

# average read length (bp)

453

485

493

482

# singletons

421

541

429

198

# clusters

155

264

194

157

# putative transcripts

576

805

623

355

Avg cluster size

3.87

2.46

3.55

6.4

Largest cluster

81

15

59

63

# clusters with 2 ESTs

113

210

146

62

# clusters with 3 ESTs

19

29

17

25

# clusters with 4–5 ESTs

10

20

16

22

# clusters with 6–10 ESTs

6

2

7

22

# clusters with > 10 ESTs

7

3

8

26

Gene discovery

0.56

0.68

0.56

0.30

Gene diversity

0.41

0.46

0.38

0.16

# (%) with significant* SwissProt hits**

308 (53%)

415 (52%)

296 (48%)

100 (28%)

# (%) with significant* trembl hits**

328 (57%)

439 (55%)

309 (50%)

110 (31%)

# (%) with no hits

242 (42%)

363 (45%)

309 (50%)

243 (68%)

  1. * Reads that are submittable and > 100 bp. ** Threshold for Blastx significance = 1e-10. Gene discovery is defined as the number of different "genes" each library contributed, divided by library size. Gene Diversity is defined as the number of singletons in each library divided by library size [22].