Table 6 Primer/probe list for UT-quantitative PCR and UT-digital PCR
From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
Primers for Standard 454 libraries: | |
|---|---|
Forward: | 5'-CCATCTCATCCCTGCGTGTC-3' |
Reverse: | 5'-CCTATCCCCTGTGTGCCTTG-3' |
UTBP-1: | 5'-GGCGGCGACCATCTCATCCCTGCGTGTC-3' |
Primers for 454 MID/Paired end libraries: | |
Forward: | 5'-GCCTCCCTCGCGCCATCAG-3' |
Reverse: | 5'-GCCTTGCCAGCCCGCTCAG-3' |
UTBP-2: | 5'-GGCGGCGAGCCTCCCTCGCGCCATCAG-3' |
Primers for Solexa libraries: | |
Forward: | 5'-ACACTCTTTCCCTACACGA-3' |
Reverse: | 5'-CAAGCAGAAGACGGCATA-3' |
UTBP-3: | 5'-GGCGGCGAACACTCTTTCCCTACACGA-3' |
Universal probe sequence: | |
UPL#149 | 5'-CCGCCGCT-3' |