Figure 5

All three predicted members of the Yfr2 family of ncRNAs are expressed in Synechocystis 6803. The three predicted ncRNAs belonging to cluster 219 are expressed in Synechocystis 6803. The ncRNA Yfr2a is ~80 nt long. All initiation sites of transcription were mapped by 5' RACE (grey arrows). The transcriptional start site of Yfr2a was mapped unambiguously to position 1558975 (complementary strand), 93 nt downstream the end of the trxA (sll1980) open reading frame. Yfr2b and Yfr2c are initiated at positions 2730523 and 3398352 (complementary strand), respectively. All three ncRNAs are very strongly expressed in Synechocystis 6803. However, the fact that it was not possible to map a specific initiation site of transcription for the genes slr0199 and sll1477 indicates their occasional or standard cotranscription with Yfr2b and Yfr2c. This assumption is further supported by some RNA fragments of higher molecular weight hybridizing with the Yfr2b probe. All labels are as in Fig. 4.