Figure 2

RT-PCR validation of splicing events identified by exon array. PCR products were derived using forward/reverse primers to amplify the flanking regions indicated under the gene name. The mobility changes are identified on the right side ("Incl" for exon inclusion; "Skip" for exon skipping). The 18S rRNA was used as a control. The primer sequences are available in an online Excel table (See Additional file 5).