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Figure 5 | BMC Genomics

Figure 5

From: Detection of genomic deletions in rice using oligonucleotide microarrays

Figure 5

Identification of a cytochrome P450 family member as a candidate for Spl1. Candidate genes located in the Spl1 region by array hybridization (Figure 4) were screened for SNPs in an EMS-generated mutant showing the spl1 phenotype by TILLING. (a) Detection of heteroduplex by TILLING between DNA for the rice mutant E16923 and wild type parent IR64 PCR products specific for LOC_Os12g16720 (a cytochrome P450 family member). Lanes 1 and 2 are CEL1 treatments of IR64 and E16923 amplicons, respectively. Lane 3 shows the activity of CEL1 enzyme on a heteroduplex generated between IR64 and E16923 amplicons. (b) Sequencing the amplified cytochrome P450 family member from E16923 confirmed the presence of a SNP at position 290 that resulted in a stop codon. Sequence data from two DEB mutants, D1137 and D2943, showing the spl1 phenotype revealed SNPs in LOC_Os12g16720 that caused amino acid changes.

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