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Table 1 Assessment of the cDNA library normalization by sequence count

From: A transcriptional sketch of a primary human breast cancer by 454 deep sequencing

Reference Gene1

454 Reads mapped to the genome (194,806)

UniGene ESTs

(39,700)

Probability of differential expression between the libraries

ACTB

11

187

Prob > 0.999

GAPDH

31

225

Prob > 0.999

HPRT1

7

0

0.5 < Prob < 0.6

  1. 1 ACTB and GAPDH are abundantly expressed housekeeping genes, while HPRT1 is expressed at low levels.
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BMC Genomics

ISSN: 1471-2164

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