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Figure 4 | BMC Genomics

Figure 4

From: Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli

Figure 4

Effects of GcvB sncRNA and OppA protein on the expression of RpoS in a chromosomal rpoS-lacZ reporter strain. (A) Effects of deleting gcvB on the expression of an rpoS-lacZ reporter gene fusion in E. coli MG1655. (B) Effect of GcvB overproduction on the expression of the rpoS-lacZ fusion. GcvB production was driven by its native promoter on a pET32a plasmid construct. (C) Effect of deleting oppA on the expression of the rpoS-lacZ reporter. Beta-galactosidase activities due to translation of chromosomal lacZ fusions were normalized to those of MG1655 carrying an rpoS-lacZ fusion with or without an empty vector (controls). Error bars represent standard deviation (* P-value < 0.05).

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