Table 5 Primers used for Real time PCR validation
From: Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos
Genes | Primer sequences and positions (5' - 3') | Fragment size (bp) | Accession Number |
|---|---|---|---|
GAPDH_F | TGCTGGTGCTGAGTATGTGGT         (333–354) | 295 | [GenBank:XM_865742] |
GAPDH_R | AGTCTTCTGGGTGGCAGTGAT         (627–648) |  |  |
DNMT3A_F | CTGGCTCTTTGAGAATGTGGTG    (2372–2394) | 236 | [GenBank:XM_867643] |
DNMT3A_R | TCACTTTGCTGAACTTGGCTATT    (2607–2630) |  |  |
DNMT3B_F | GGGAAGGAGTTTGGAATAGGAG     (698–720) | 417 | [GenBank:NM_181813] |
DNMT3B_R | CTCTGGTTGCTTGTTGTTAGGTT     (1114–1137) |  |  |
IGF2R_F | AACCAGGTGATTTAGAAAGTGCC  (1939–1962) | 397 | [GenBank:NM_174352] |
IGF2R_R | CGCTTCTCGTTATTGTAGGGTG      (2335–2357) |  |  |
PLAC8_F | TGTTTCACAGCCAGGTTACAGC        (168–190) | 200 | [GenBank:NM_001025325] |
PLAC8_R | GGGTCCGATACATTGTCCTCAT         (367–389) |  |  |
PGR_F | TAAATGACCAGCAAGCAGAAACT     (562–585) | 394 | [GenBank:XM_613908] |
PGR_R | GGTAATTGTGCAGCAATAACCTC      (955–978) |  |  |
BIT1_F | CGGAGCCAGAGGAAGAATGA              (75–95) | 445 | [GenBank:NM_001034519.1] |
BIT1_R | TGCTTGTAGGCAGAAACAGCA          (519–530) |  |  |
HMGN3_F | GTTCCAGCCCGTTGCTTTAC                 (22–42) | 355 | [GenBank:NM_001034504.1] |
HMGN3_R | GACCATTCATTCTCCCTCGTTAG        (376–399) |  |  |
HSPA1A_F | CACGATGTTGATCCTGTGGG               (86–106) | 380 | [GenBank:NM_174550.1] |
HSPA1A_R | CACCTTAGGCTTGTCTCCGTC            (465–487) |  |  |
NGDN_F | GTGAGAATGACCCACTCCGTT          (403–424) | 397 | [GenBank:NM_001046459] |
NGDN_R | TCCCGCTTGCTGACACTTAA              (799–819) |  |  |
FBXO9_F | GCAGACGGCAGGAGTAGACAC        (231–252) | 445 | [GenBank:NM_001034412.1] |
FBXO9_R | ACAAGTTGCATAGCCCTACGAT        (675–697) |  |  |
GNAI2_F | TCCAGACAACTGCCAACATCA       (1978–1999) | 215 | [GenBank:XM_589440.3] |
GNAI2_R | CAAACCAGGTGAACAATTCCATA   (2192–2215) |  |  |
PALLD_F | AGGTTGACCTACGAGGAAAGGA    (2071–2092) | 292 | [GenBank:XM_869983.2] |
PALLD_R | ATGTGAACGTCGCAGGCATA          (2362–2382) |  |  |
NFYA_F | CGGGCTAAATTAGAAGCAGAAG       (998–1020) | 311 | [GenBank: NM_001014956.1] |
NFYA_R | AGGGCAGAATGTGATCGTCAG        (1308–1329) |  |  |
GATM_F | ATTGGCTGCTCAGGGAAAGT              (824–844) | 262 | [GenBank: NM_001045878.1] |
GATM_R | ACATGGTCGGTCAGGGTTG            (1085–1104) |  |  |
TASPASE1_F | CAAGACTCATATTTCCAGACTCCC    (1145–1169) | 264 | [GenBank: NM_001034577.1] |
TASPASE1-R | CCAAGCACTAACTACAGCAGCAC    (1408–1431) |  |  |