Figure 4
From: Activation of p53-regulated pro-apoptotic signaling pathways in PrP-mediated myopathy
Real-time PCR analysis of mdm2 and p53. qRT-PCR analysis of mdm2 (grey) and p53 (black) gene expression in RNA samples from Tg(HQK) PrP over-expressing mice relative to similarly treated wild-type control mice. Measurements of relative gene expression for 5 time points (over 4–60 days) in mice following treatment with 6 g Dox/kg food. Total RNA was extracted from skeletal muscles (quadriceps) from the hind legs and subjected qRT-PCR analysis. Results represent the mean ± s.e.m. of triplicate measurements performed. ** p < 0.01; *** p < 0.001.