Figure 1

Reference microarray hybridization schema and infection time course experimental design. (A) The reference hybridization schema used for the microarray experiments. The common reference RNA pool was generated by combing equal quantities of all samples in the study, which included RNA from pre- and post-infection time points. Each arrow represents a single array hybridized with one experimental sample labelled with Alexa Fluor^® 555 (green) and one common reference RNA sample labelled with Alexa Fluor^® 647 (red). (B) The experimental design used for the trypanosome infection time course. The double-headed arrows show the gene expression contrasts that were catalogued across the infection time course for the trypanotolerant N'Dama breed (blue) and the trypanosusceptible Boran breed (orange). Gene expression contrasts between breeds at each time point are shown as double-headed purple arrows. Days post infection, dpi. The arrow colour coding for the gene expression contrasts corresponds to the colour coding of the graphs shown in Figs. 2 and 3.