Figure 4

Real time qRT-PCR results for nine selected genes from trypanotolerant N'Dama and trypanosusceptible Boran cattle across the trypanosome infection time course. Data analysis was carried out using the standard 2^-ΔΔCt method [55]. All C_t values were corrected using the housekeeping gene PPIA and the N'Dama group at time point 0 was used as the calibrator. Each point on the graph (common log scale) represents the mean fold change in gene expression relative to pre-infection levels ± SEM. Genes shown are: GFM1, CD19, RSF1, STX7, SCAMP1, CYBB, GMPS, MAP4K3 and LTB.