Figure 5

Real time qRT-PCR results for 14 selected genes from trypanotolerant N'Dama and trypanosusceptible Boran cattle across the trypanosome infection time course. Data analysis was carried out using the standard 2^-ΔΔCt method [55]. All C_t values were corrected using the housekeeping gene PPIA and the N'Dama group at time point 0 was used as the calibrator. Each point on the graph (common log scale) represents the mean fold change in gene expression relative to pre-infection levels ± SEM. Genes shown are: CD3E, DUSP1, IFIT2, LTBR, PIR, FOS, CD14, CEBPB, TIMP3, ICAM3, MAPK14, SEPP1, NFIL3, and SLC40A1.