Table 4 Bovine oligonucleotide primers used for real time qRT-PCR.
Gene symbol | Gene name | Primer sequence (5'-3')a | Amplicon size | Primer concentration |
|---|---|---|---|---|
PPIA | Peptidylprolyl isomerase A | F-CATACAGGTCCTGGCATCTTGTCCÂ R-CACGTGCTTGCCATCCAACCÂ Â Â Â Â | 108 bp | 300 nM |
BAFF | B cell activating factor, TNFSF13B | F-Â GGAGATGAACTCCAACTGGCAAÂ Â R-Â GTGAGCAGGTCACAGAAGCTTCÂ Â | 102 bp | 100 nM |
CD3E | T-cell receptor CD3 epsilon chain | F-Â CTGGTGTATTACTGGAGCAAGAGCÂ R-Â GGGCTCATAGTCTGGATTGGÂ Â Â Â Â | 132 bp | 300 nM |
CD14 | CD14 antigen | F-Â GGTCTCTCAATTTGTCGTTCGCÂ Â Â R-Â GATTTCCGTCCAGAGTCAGGÂ Â Â Â Â | 153 bp | 900 nM |
CD19 | CD19 antigen | F-Â CTTGGGTCCCAATCCTATGAGGÂ Â Â R-Â CATGATTAGCACCAGGCTGGÂ Â Â Â Â | 91 bp | 300 nM |
CEBPB | CCAAT/enhancer binding protein beta | F-Â TGCAACGCCTGGTGGTCTGGÂ Â Â Â Â R-Â CAGCCAAGCAGTCCGCCTCGÂ Â Â Â Â | 111 bp | 300 nM |
CTSS | Cathepsin S | F-Â AGGAAAGCTGGTGTCTCTGAGTGCÂ R-Â TGTCATGAAGCCGCCATTGCÂ Â Â Â Â | 91 bp | 300 nM |
CYBB | Cytochrome b-245, beta polypeptide | F-Â TGGGAACCCTCCTATGACTTGGÂ Â Â R-Â TGACCACCTTGGTGATGACCÂ Â Â Â Â | 116 bp | 300 nM |
DUSP1 | Dual specificity phosphatase 1 | F-Â CCTTGATCAACGTCTCTGCGÂ Â Â Â Â R-Â TCCACCAGCATTCTTGATGGÂ Â Â Â Â | 152 bp | 100 nM |
FOS | Murine FBJ osteosarcoma viral (v-fos) oncogene homolog | F-Â CAATAACTACTGTGTTCCTGGCÂ Â Â R-Â TCAGACCACCTCAACAATGCÂ Â Â Â Â | 186 bp | 300 nM |
GBP4 | Guanylate binding protein 4-like | F-Â CCCATGCAAAGACCAAGACCÂ Â Â Â Â R-Â ACTGATCCACTGTTGATGGCÂ Â Â Â Â | 103 bp | 300 nM |
GFM1 | Elongation factor G1 | F-Â GTTCCTTTGTGACAACCTGGÂ Â Â Â Â R-Â CTACTTCTCTGCTCTCTGCGÂ Â Â Â Â | 159 bp | 300 nM |
GMPS | Guanine monphosphate synthetase | F-Â AAGTCTGCACTCACTGAATGCÂ Â Â Â R-Â TAAGAGATTCCCAGTCAGGCÂ Â Â Â Â | 125 bp | 300 nM |
GZMB | Granzyme B precursor | F-Â ACCTACGCTGACACACTACAGGÂ Â Â R-Â ACATTGTCACACACGAGAGGÂ Â Â Â Â | 173 bp | 300 nM |
ICAM3 | Intercellular adhesion molecule 3 | F-Â GATGATGGACGTTCAAGGTCGÂ Â Â Â Â R-Â AAGACGTACACTGAGGCTGCÂ Â Â Â Â | 105 bp | 300 nM |
IFIT2 | Interferon-induced protein with tetratricopeptide repeats 2 | F-Â CCAGTATGTCAAAGTCCTCCÂ Â Â Â Â R-Â ATAGCAAGGTCCAAGTCACCÂ Â Â Â Â | 165 bp | 100 nM |
LTB | Lymphotoxin-beta isoform a | F-Â CTACAATAAACCCTCCACGGÂ Â Â Â Â R-Â CAGACAGGACATCTCCATCCÂ Â Â Â Â | 117 bp | 900 nM |
LTBR | Lymphotoxin beta receptor | F-Â CCAGGTCAGGAACATGTGTGÂ Â Â Â Â R-Â GCCTCTGGTCTCATTTCTGCÂ Â Â Â Â | 176 bp | 900 nM |
LYZ | Lysozyme | F-Â CTCAGTCAACATGAAGGCTCTCGÂ Â R-Â ACACAACCAGTTTGCCAGGCÂ Â Â Â Â | 154 bp | 900 nM |
MAP4K3 | Mitogen-activated protein kinase kinase kinase kinase 3 | F-Â TTTGAGTCACAGAATAGCGGÂ Â Â Â Â R-Â TGAACTGATTCCCTCTATGGÂ Â Â Â Â | 155 bp | 900 nM |
MAPK14 | Mitogen-activated protein kinase 14 | F-Â GCTGTCGACCTGCTGGAGAAGATGC R-Â TCGTCGTCAGGATCGTGGTACTGGÂ | 110 bp | 300 nM |
NCR3 | Natural cytotoxiciy triggering receptor 3 | F-Â TAGAGACGCCAAGAGTGGAAGGÂ Â Â R-Â AACCTCTCGCGAAGATAAGCÂ Â Â Â Â | 158 bp | 300 nM |
NFE2L2 | Nuclear factor (erythroid-derived 2)-like 2 | F-Â CAACCTTTGTCGTCATCACAGGÂ Â Â R-Â GGGAATGGGATATGGAGAGCÂ Â Â Â Â | 194 bp | 900 nM |
NFIL3 | Nuclear factor, interleukin 3 regulated | F-Â CCAGAAGTGAATTCCTCTGCCTTGC R-Â TTAGACGTCATATCAACAGGCGAGG | 134 bp | 100 nM |
PIR | Pirin | F-Â TTAAATTGGACCAAGGAGCGÂ Â Â Â Â R-Â ACAAAGTGGCTTCTCTCAGGÂ Â Â Â Â | 196 bp | 900 nM |
RAB35 | Member RAS oncogene family | F-Â GAACGATCAGGGTCTTCTGCÂ Â Â Â Â R-Â GATGTGATTGTCCAGTCAGGÂ Â Â Â Â | 173 bp | 900 nM |
RSF1 | Remodelling and spacing factor 1 | F-Â TGCATTCCAAATCTGTGAGGÂ Â Â Â Â R-Â GCAACAATACGGACTTGAACÂ Â Â Â Â | 157 bp | 900 nM |
SCAMP1 | Secretory carrier-associated membrane protein 1 | F-Â TACAGACCACTTTACGGAGCÂ Â Â Â Â R-Â GAAATCCAACCACAGTTACCÂ Â Â Â Â | 140 bp | 300 nM |
SEPP1 | Selenoprotein P, plasma, 1 | F-Â CCCTACTTCAGGTCTTCATCACCÂ Â R-Â TGCATCTCTTTCGTCAGAGCÂ Â Â Â Â | 143 bp | 300 nM |
SLC40A1 | Solute carrier family 40 (iron-regulated transporter), member 1 | F-Â ATAATACAGTGAAGGCACGAGGÂ Â Â R-Â CAAGACAGAGATCCTGAAGGÂ Â Â Â Â | 130 bp | 300 nM |
STX7 | Syntaxin-7 | F-Â ATCTGGTGGTTTTCCTGAGGÂ Â Â Â Â R-Â TCTTCATCCTGCAACTGAGCÂ Â Â Â Â | 93 bp | 300 nM |
TIMP3 | Tissue inhibitor of metalloproteinase 3 | F-Â ATCTCCCAGACTCTCCTTCCÂ Â Â Â Â R-Â ACAGTTGGCTTTTCAGCAGCÂ Â Â Â Â | 91 bp | 300 nM |
XDH | Xanthene dehydrogenase | F-Â CTCCGTACAGACATTGTCATGGÂ Â Â R-Â GGAATAGTGTAGCTCCTCCAGGÂ Â Â | 123 bp | 300 nM |