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Table 3 Segregation analysis of EST-SSR markers in a Silene cross.

From: A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping

Locus Male × female Genotype of progeny Expected ratio Observed ratio X2
SL_eSSR01 A/B × A/B A/A:A/B:B/B 1:2:1 60:5:17 108.31*
SL_eSSR02 A/B × A/B A/A:A/B:B/B 1:2:1 17:33:33 9.65
SL_eSSR03 A/Ba × A/C A/A:A/C:A/B:B/C 1:1:1:1 18:8:34:13 19.35*
SL_eSSR04 A/B × A/B A/A:A/B:B/B 1:2:1 27:52:2 21.96*
SL_eSSR05 A/B × C/D A/C:A/D:B/C:B/D 1:1:1:1 21:5:25:29 16.60*
SL_eSSR06 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 8:25:21:27 8.55
SL_eSSR07 A/B × A/B A/A:A/B:B/B 1:2:1 17:43:21 0.70
SL_eSSR08 A/B × A/B A/A:A/B:B/B 1:2:1 22:23:32 15.07*
SL_eSSR09 A/B × A/B A/A:A/B:B/B 1:2:1 4:32:19 9.65
SL_eSSR10 A/B × A/B A/A:A/B:B/B 1:2:1 19:43:21 0.20
SL_eSSR11 A/B × C/Da A/C:A/D:B/C:B/D 1:1:1:1 21:13:28:21 5.43
SL_eSSR12 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 27:16:21:19 3.12
SL_eSSR13 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 26:13:22:18 4.70
SL_eSSR14 A/B × A/B A/A:A/B:B/B 1:2:1 24:43:19 0.58
SL_eSSR16 A/B × A/B A/A:A/B:B/B 1:2:1 16:45:17 1.87
SL_eSSR17 A/B × A/B A/A:A/B:B/B 1:2:1 8:53:18 11.92
SL_eSSR20 A/B × A/B A/A:A/B:B/B 1:2:1 15:28:15 0.06
SL_eSSR21 A/B × A/A A/B × A/A 1:1 57:22 15.50*
SL_eSSR22 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 5:24:19:23 13.00
SL_eSSR24 A/B × A/A A/B × A/A 1:1 47:11 24.27*
SL_eSSR25 A/B × C/D A/C:A/D:B/C:B/D 1:1:1:1 21:19:20:23 0.42
SL_eSSR26 A/B × A/B A/A:A/B:B/B 1:2:1 31:36:19 0.31
SL_eSSR27 A/B × A/A A/B × A/A 1:1 41:21 6.45
SL_eSSR28 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 22:21:21:13 2.74
SL_eSSR29 A/B × A/C A/A:A/C:A/B:B/C 1:1:1:1 22:23:21:18 0.66
  1. *Significant deviation from expected Mendelian ratio after Bonferroni correction (P < 0.002)
  2. a indicates an inferred null allele
  3. 90 F2 offspring from a cross between Silene latifolia and S. dioica were genotyped and segregation ratios tested.