Table 1 Tabulation of S. aureus essential genes by category.
Category | Number of essential genes in S. aureus | Number of S. aureus essential genes with an essential orthologue in B. subtilis | Number of B. subtilis essential genes with no essential orthologue in S. aureus |
|---|---|---|---|
DNA metabolism | 27 | 22 | 3 |
DNA replication | 16 | 16 | 0 |
DNA packaging and segregation | 11 | 6 | 3 |
RNA metabolism | 25 | 14 | 2 |
Basic transcription machinery | 4 | 4 | 0 |
RNA modification | 10 | 7 | 2 |
RNA regulation | 11 | 3 | 0 |
Protein synthesis | 102 | 86 | 7 |
Ribosomal proteins | 49 | 47 | 4 |
tRNA synthetase | 22 | 20 | 1 |
tRNA modification | 2 | 2 | 0 |
Translation factors | 13 | 10 | 1 |
Protein folding | 5 | 2 | 0 |
Protein modification | 3 | 0 | 0 |
Protein translocation | 8 | 5 | 1 |
Cell envelope | 66 | 43 | 22 |
Lipids | 15 | 11 | 0 |
Phospholipids | 4 | 4 | 1 |
Cell wall/amino sugar | 3 | 3 | 3 |
Diaminopimelate biosynthesis | 0 | 0 | 4 |
Peptidoglycan biosynthesis | 17 | 10 | 2 |
Teichoic acid biosynthesis | 12 | 8 | 0 |
Cell division | 12 | 7 | 0 |
Cell shape | 0 | 0 | 3 |
Na/H transporter | 0 | 0 | 9 |
Cell envelope – other | 3 | 0 | 0 |
Carbon metabolism | 20 | 9 | 0 |
Glycolysis | 11 | 9 | 0 |
Pentose phosphate | 4 | 0 | 0 |
Intermediary metabolism | 3 | 0 | 0 |
Regulation | 2 | 0 | 0 |
Respiratory pathways | 11 | 4 | 9 |
Isoprenoid/Mevalonate biosynthesis | 8 | 1 | 2 |
Menaquinone biosynthesis | 1 | 1 | 7 |
Thioredoxin | 2 | 2 | 0 |
Nucleotides | 12 | 8 | 2 |
Purine biosynthesis | 3 | 2 | 2 |
Purine metabolism | 1 | 0 | 0 |
Purine/Pyrimidine biosynthesis | 3 | 3 | 0 |
Pyrimidine biosynthesis | 5 | 3 | 0 |
Cofactors | 24 | 13 | 2 |
Acetyl CoA/CoA | 5 | 1 | 0 |
Folate | 6 | 1 | 2 |
NAD biosynthesis | 4 | 4 | 0 |
SAM | 1 | 1 | 0 |
Fe-sulphate cluster | 7 | 6 | 0 |
Riboflavin biosynthesis | 1 | 0 | 0 |
Other/unknown | 64 | 14 | 2 |
Amino acid transporter | 1 | 0 | 0 |
GTP binding | 6 | 6 | 0 |
Other | 4 | 3 | 1 |
Unknown | 53 | 5 | 1 |