Table 3 Comparison of fold-changes for selected genes between 23 and 35 DAP in B. napus seeds measured by LongSAGE and Real-time RT-PCR.
A. thaliana gene locus | Gene | Cumulated counts,35:23 DAP LongSAGE (non-normalized) | Fold-change 35:23 DAP LongSAGE (normalized) | Fold-change 35:23 DAP Real-time RT-PCR | B. napus EST contigs assembled by CAP3 |
|---|---|---|---|---|---|
AT2G40890 | Coumarate 3-hydroxylase (C3H) | 2:2 | 1.9 | 2.0 4.1 | Contig 1 from 5 ESTs Contig 2 from 2 ESTs |
AT5G48930 | Hydroxycinnamoyl-Coenzyme A shikimate/quinate hydroxycinnamoyl-transferase (HCT) | 5:3 | 3.2 | 2.3 | 1 EST |
AT1G15950 | Cinnamoyl-CoA reductase 1 (CCR1) | 2.2:1 | 4.3 | 0.9 | Contig from 4 ESTs |
AT2G23910 | Cinnamoyl-CoA reductase-related (CCR1-related) | 0:0 | - | 1.2 1.7 | Contig 1 from 22 ESTs Contig 2 from 13 ESTs |
AT3G19450 | Cinnamoyl alcohol dehydrogenase (CAD1) | 8:9.5 | 1.6 | 2.1 | Contig 3 from 4 ESTs |
AT4G27140 AT4G27150 AT4G27160 AT4G27170 | 2S seed storage proteins (napin) | 669.1:3.4 560.1:1.9 575.3:1.9 636.5:7.9 | 374.1 564.4 579.6 155.0 | 1224 | Contig from SAGE primer-amplified cloned 3'-terminal napin gene fragments |