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Table 6 Comparison of transcript abundance of representative maize genes estimated by GS20–454 sequencing and qPCR.

From: Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing

Gene locus matched

Accession (ZMGI)

Ra454 reads

Ct qPCR (mean ± SD)

Similar to UP|LIRP1_ORYSA (Q03200) Light-regulated protein precursor

TC327885

2.11

11.7808 ± 0.09

Similar to UP|PSAE_HORVU (P13194) Photosystem I reaction center subunit IV, chloroplast precursor (PSI-E)

TC361477

0.74

13.1666 ± 0.05

UP|Q41754_MAIZE (Q41754) Ubiquitin

TC369342

0.49

14.6629 ± 0.10

UP|TBA6_MAIZE (P33627) Tubulin alpha-6 chain (Alpha-6 tubulin)

TC326717

0.32

14.0284 ± 0.03

Homologue to GB|BAD33626.1|50726105|AP005579 Polyubiquitin 2

TC342043

0.32

16.3357 ± 0.04

Homologue to UP|Q41772_MAIZE (Q41772) Cytosolic ascorbate peroxidase, complete

TC364641

0.22

16.5425 ± 0.08

Similar to UP|SODC2_MESCR (O49044) Superoxide dismutase [Cu-Zn]2

TC327155

0.08

17.9920 ± 0.08

Similar to UP|Q6YIH2_ORYSA (Q6YIH2) OsCDPK protein

TC327230

0.06

18.5091 ± 0.13