Analysis of expression in the Anopheles gambiae developing testes reveals rapidly evolving lineage-specific genes in mosquitoes

Table 1 Basic sequence characteristics of the Anopheles gambiae genes isolated during this study and their orthologs from Aedes aegypti and Culex quinquefasciatus.

Species	Length (nt)	5'UTR (nt)	Coding region (nt)	3'UTR (nt)	Protein length (aa)	Identity//similarity¹ (%)	Gene status²
*AgRopn1l*
An. gambiae	2391–2506³	29–144³	2040	319	679		unannotated
Ae. aegypti			2166		721	54.2/65.3	14993
Cx. quinquefasciatus			2091		696	74.7/83.8	07290
*AgDzip1l*
An. gambiae	2623–2707³	76–160³	2547	48	848		01165
Ae. aegypti			2502		833	46.5/58.1	unannotated
Cx. quinquefasciatus			2328		775	63.5/74.2	11569
*Ams*
An. gambiae	1356	39	1227	90	408		unannotated
Ae. aegypti			nd⁴		nd	13.8/30.8⁵	unannotated
Cx. quinquefasciatus			nd		nd	70.8/89.9⁵	unannotated
*mts*
An. gambiae	1122	108	897	117	295		unannotated
Ae. aegypti			1102		346	25.1/36.1	09638
Cx. quinquefasciatus			912		303	45.8/54.9	00571
*AAms*
An. gambiae	3724	87	3567	157	1188		unannotated
Ae. aegypti			4707		1568	22.6/38.5	unannotated
Cx. quinquefasciatus			5022		1673	36.1/50.4	unannotated

¹ Protein sequence identity/similarity calculated after exclusion of gapped regions. Values for the An. gambiae/Ae. aegypti comparisons are given in the rows corresponding to Aedes aegypti; these values are also representative of the identity/similarity between An. gambiae and Cx. quinquefasciatus. The identity/similarity between Ae. aegypti and Cx. quinquefasciatus proteins is given in the Cx. quinquefasciatus rows.
² The Ensembl gene ID (abridged by omitting the species codes AGAP0, AAEL0, and CPIJ0 for An. gambiae, Ae. aegypti, and Cx. quinquefasciatus; respectively) is given for genes with complete (bold) or partially (italic) predicted sequence in the recent VectorBase gene builds (An. gambiae: AgamP3.4; Ae. aegypti: AaegL1.1; Cx. quinquefasciatus: CpipJ1.0_5); genes that do not match any gene predictions are marked as unannotated.
³ Several alternative transcription start sites identified.
⁴ nd – not determined
⁵ Data based on a portion of a gene sequence; see text for details.

ISSN: 1471-2164