Figure 4

Quantitative strand-specific RT-PCR analysis of SAS pair expression in breast cancer cell lines and solid primary breast tumours. cDNAs of 15 breast cancer cell lines and 10 primary breast tumours (BC) were analyzed using the ΔΔCT relative quantification real-time qPCR. Red bars represent sense transcripts, green bars the corresponding antisense transcripts. Analysis of qPCR data was performed using the immortalised luminal cell line (226L) as comparator for all the breast cell lines and solid primary breast tumours (indicates as BC). ACTB was used as endogenous control throughout all analysis. ΔΔCT relative quantification data are expressed as mean fold changes across samples together with 95% confidence intervals.