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Table 7 Rate of false expression microarray results based on QPCR gold standard by amplification technique

From: A comparison of RNA amplification techniques at sub-nanogram input concentration

Method

Quantitiy of Input

# False

% FER

Modified T7

500 pg

5

13.5

 

500 pg

5

13.5

 

500 pg

6

19

 

1 ng

6

19

 

1 ng

5

13.5

 

Mean

5.4

14.6

Arcturus

250 pg

6

19

 

250 pg

6

19

 

500 pg

4

10.8

 

1 ng

4

10.8

 

Mean

5

13.5

Balanced PCR

500 pg

1

2.7

 

667 pg

5

13.5

 

667 pg

4

10.8

 

3.3 ng

5

13.5

 

3.3 ng

6

19

 

Mean

4.2

11.3