From: A comparison of RNA amplification techniques at sub-nanogram input concentration
Method | Quantitiy of Input | # False | % FER |
---|---|---|---|
Modified T7 | 500 pg | 5 | 13.5 |
 | 500 pg | 5 | 13.5 |
 | 500 pg | 6 | 19 |
 | 1 ng | 6 | 19 |
 | 1 ng | 5 | 13.5 |
 | Mean | 5.4 | 14.6 |
Arcturus | 250 pg | 6 | 19 |
 | 250 pg | 6 | 19 |
 | 500 pg | 4 | 10.8 |
 | 1 ng | 4 | 10.8 |
 | Mean | 5 | 13.5 |
Balanced PCR | 500 pg | 1 | 2.7 |
 | 667 pg | 5 | 13.5 |
 | 667 pg | 4 | 10.8 |
 | 3.3 ng | 5 | 13.5 |
 | 3.3 ng | 6 | 19 |
 | Mean | 4.2 | 11.3 |