From: Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens
Mutagenesis | Selection medium (electron donor/acceptor) |
---|---|
1. Deletion and replacement of the rpoN gene by double-crossover (Figure 1b) | Acetate/fumarate |
 | Acetate/Fe(III) citrate |
 | Acetate/Fe(III) citrate amended with glutamine |
 | Acetate/Fe(III) citrate amended with glutamate |
 | Acetate/Fe(III) citrate amended with both glutamine and glutamate |
2. Deletion and replacement of the 5'-end of the rpoN gene by double-crossover (Figure 1b) | Acetate/fumarate |
 | Acetate/Fe(III) citrate |
3. Deletion and replacement of the 3'-end of the rpoN gene by double-crossover (Figure 1b) | Acetate/fumarate |
 | Acetate/Fe(III) citrate |
4. Deletion and replacement of the upstream intergenic region of the rpoN gene by double-crossover (Figure 1a) | Acetate/fumarate |
5. Deletion and replacement of the downstream intergenic region of the rpoN gene by double-crossover (Figure 1a) | Acetate/fumarate |
6. Integration of a linear DNA fragment on the chromosome, providing another copy of the rpoN gene on another location of the chromosome (Figure 1c) | Acetate/fumarate |
 | Acetate/Fe(III) citrate |
7. Integration of a linear DNA fragment on the chromosome, providing another copy of the rpoN gene in trans, which is either constitutively expressed by a lac promoter, or is IPTG inducible (Figure 1d) | Acetate/fumarate |
 | Acetate/Fe(III) citrate |
 | Acetate/Fe(III) citrate amended with both glutamine and glutamate |