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Table 1 Microarray and qRT-PCR comparison

From: Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility

 

Microarray Results

qRT-PCR Results

 

MEF STMN1-/-

   

STMN2

-2.44

1.27

 

STMN3

N/A

N/A

 

STMN4

2.26

8.06

*

Tubb2a/Class IIa

1.40

1.80

*

Tubb2b/Class IIb

1.54

2.64

*

Tubb2c/Class IVb

-1.35

-0.57

*

Tubb3/Class III

1.22

1.78

*

Tubb6/Class V

-1.31

-0.57

*

  1. Magnitude and direction of gene expression changes found using microarray analysis compared to qRT-PCR with designed primers. Only those tubulin isotypes whose expression was significantly changed, as measured by microarray with FDR < 0.05, are shown compared to qRT-PCR (*, p-value < 0.005). Data are given as fold change in STMN-/- MEFs relative to STMN+/+ MEF.