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Table 1 Statistics of small RNA sequences from the Brachypodium NC and WC libraries.

From: Deep sequencing of Brachypodium small RNAs at the global genome level identifies microRNAs involved in cold stress response

  Sequences generated Unique sequences
NC library   
   Raw reads 5,339,385  
   Low quality reads removed 4,702,002  
   Adaptors removed 3,816,932  
   Sequences < 18 nt removed 3,741,194  
   Sequences matching Brachypodium draft genome 2,603,872  
   Non-coding RNA exact matches removed 2,248,474 711,207
   Match known miRNAs 851,945 27
   Genome-matched small RNAs with predicted hairpin structures 41,155 235
WC library   
   Raw reads 5,452,742  
   Low quality reads removed 4,720,226  
   Adaptors removed 4,314,516  
   Sequences < 18 nt removed 3,585,498  
   Sequences matching Brachypodium draft genome 2,958,093  
   Non-coding RNA exact matches removed 1,264,919 423,463
   Match known miRNAs 337,863 27
   Genome-matched small RNAs with predicted hairpin structures 12,750 218
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BMC Genomics

ISSN: 1471-2164

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