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Table 3 Relative gene expression of the non-reference genes in D. magna after normalisation in the fish-kairomone experiment.

From: Target gene approaches: Gene expression in Daphnia magna exposed to predator-borne kairomones or to microcystin-producing and microcystin-free Microcystis aeruginosa

 

Relative expressiona

Gene

Calibrator

Vertebrate predator

SDb

18S

1.0000 ± 0.0123

2.2449 ± 0.0278

0.8803

28S

1.0000 ± 0.0519

1.8555 ± 0.0873

0.6049

cyclophilin

1.0000 ± 0.0012

2.9216 ± 0.0020

1.3588

UBC

1.0000 ± 0.0046

2.0671 ± 0.0251

0.7546

  1. D. magna was raised in either a predator-free environment ('Calibrator') or in incubation water of Leucaspius delineatus (sunbleak, 'Vertebrate predator').
  2. aValues are mean of n = 3 replicates ± SD. Expression levels are displayed relative to the mean control level
  3. bSD is the variation of one reference gene across treatments