Table 5 Primer sequence information used in this study
Primers | Sequence (5'-3') | Binding region | Source | Estimated size of PCR products |
|---|---|---|---|---|
LCO1490 | GGTCAACAAATCATAAAGATATTGG | cox1 | [75] | 712 bp |
HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | cox1 | Â | Â |
16SA | CGCCTGTTTATCAAAAACAT | rrnL | [76] | 437 bp |
16SB | CCGGTTGAACTCAGATCA | rrnL | Â | Â |
Cytb-Uni5-2 | GGATCCGGHTATGTBYTVMYDTGAGG | cob | This study | 451 bp |
Cytb-Uni3-2 | GGATCCAYARRAARTAYCATTCWGG | cob | Â | Â |
CO2-Uni5 | GGWCATCAWTGRTATTGRAVWTATGA | cox2 | This study | 296 bp |
CO2-Uni3 | TGATTWRCHCCACAVATWTCWGMACA | cox2 | Â | Â |
RotspCO1+425 | GGCTTCATATTGCGGGTGTCTC | cox1 | This study | 1,559 bp |
Roti16S-60 | TTAGTACGGTCAGATTACTGCAGC | rrnL | Â | Â |
Roti16S+320 | AGTTGTTTACTACCTCGATGTTGGATC | rrnL | This study | 3,082 bp |
RotaCtB-110 | AAAACGTGAAAGAGTAGGAGCACCTACTC | cob | Â | Â |
RotaCtB+260 | GTGACTTCTATTAATGATAAGGTGGAG | cob | This study | 4,668 bp |
RotaCO2-100 | CCGGGATTACAACACGATTATCTACATC | cox2 | Â | Â |
RotaCO2+180 | CGGCAGATGTTCTTCATTCTTGAGCG | cox2 | This study | 5,481 bp |
RotspCO1-265 | CGAGGAAAAGCCATATCTATCAC | cox1 | Â | Â |