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Table 4 Top 5 enriched canonical pathways among DEG with ≥ 1.5-fold due to intramammary infection1.

From: Gene network and pathway analysis of bovine mammary tissue challenged with Streptococcus uberis reveals induction of cell proliferation and inhibition of PPARγ signaling as potential mechanism for the negative relationships between immune response and lipid metabolism

   

Genes

Canonical Pathway

Up/Down

#Genes/Total2

Up-Regulated

Down-Regulated

IL-10 Signaling

19/0

19/71

BCL3*3, CCL2, FOS*, HSPA8, IL1B*, IL1R2*, IL1RN*, IL6*, IL8*,

IL10*, NFKBIA*, STAT3*, TNF*

 

LXR/RXR Activation

11/4

15/85

ARG2, CCL2, CD14*, IL1B*, IL1R2*, IL1RN*, IL6*, IL18*, LDLR*, TLR4*, TNF*

CD36*, FASN, LPL*, SCD*

IL-6 Signaling

13/0

13/94

BCL3*, CD14, FOS*, IL1B*, IL1R2*, IL1RN*, IL6*, IL8*, IL18*, NFKB1A*, STAT3*, TNF*, TNFIP6

 

Triggering receptor expressed on myeloid cells 1 (TREM1) signaling

11/0

11/69

CCL2, IL1B*, IL6*, IL8*, IL10*, IL18*, STAT3*, TLR2*, TLR4*, TNF*, TREM1

 

Glucocorticoid Receptor Signaling

17/0

17/275

ANXA1*, BCL3*, FOS*, HMOX1*, HSPA5, HSPA8, IL1B*, IL1R2*, IL1RN*, IL6*, IL8*, IL10*, SMAD3, STAT3*, TNF*, VCAM1

 
  1. 1One rear quarter of all mid-lactation Holstein cows (n = 10) was inoculated with 5,000 cfu of Streptococcus uberis (strain O140J).
  2. 2#genes/total = number of differentially expressed genes from microarray and qPCR analysis (≥ 1.5-fold change; FDR ≤ 0.06; P < 0.01) out of total # of genes associated with the canonical pathway according to Ingenuity Pathway Analysis.
  3. 3genes with * symbol = qPCR expression results for verification of genes on microarray as well as genes selected for qPCR analysis not present on microarray.