Figure 5
From: Ligand-specific sequential regulation of transcription factors for differentiation of MCF-7 cells
Effect of c-FOS siRNA on FRA-1 and FHL2 expression. (A) MCF-7 cells were treated with 10 nM HRG and mRNA expression was analyzed using qRT-PCR. Numbers on the y-axis represent the relative mRNA level; bars ± SEM. Data points were obtained from three replicas. GAPDH was used as a standard. (B) Western blot analysis. Each graph shows the densitometric analysis of blots obtained from two independent experiments. Protein signal intensity was determined by normalizing each value with tubulin-α protein. Bars ± range. Red lines, control cells; blue lines, c-FOS knockdown.