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Figure 7 | BMC Genomics

Figure 7

From: Ligand-specific sequential regulation of transcription factors for differentiation of MCF-7 cells

Figure 7

Relationship among FHL2 and ERK activity. (A) MCF-7 cells were transfected with FHL2 siRNA. FHL2 and c-FOS mRNA expression levels following 10 nM HRG treatment were analyzed by qRT-PCR. Bars ± SEM. (B) ERK phosphorylation and total protein levels were investigated by Western blot analysis. Phosphorylated protein expression levels were determined by normalizing each value with total protein. Mean and range values are indicated in the graphs. Two independent studies were performed for each experiment. Red lines, control cells; blue lines, FOSL-1/FHL2 knockdown. (C) Time-course appearance pf ERK phosphorylation and FHL2 protein and FHL2 mRNA expression. HRG-treated MCF-7 cells were lysed with Bio-Plex assay reagent. Normalized ERK phosphorylation is indicated by the red bars and FHL2 protein level determined by immunoblotting and FHL2 mRNA level as determined from the GeneChip data (from Fig. 1C) is indicated by the green and blue bars, respectively.

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