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Table 2 Amplification and bin mapping success for 857 RosCOS primer pairs.

From: Development and bin mapping of a Rosaceae Conserved Ortholog Set (COS) of markers

 

Polymorphic RosCOS

  

Amplification in TxE1

Bin mapped

Orphan COS2

Putative bin 4:183

Inconclusive segregation

Failed sequence

Monomorphic

784 (91%)

607 (78%)

36 (5%)

6 (0.8%)

8 (1%)

47 (6%)

80 (10%)

  1. 1PCR amplification success in the TxE mapping population was visualized on agarose gels.
  2. 2RosCOS markers that mapped in bins that are not reported (orphan bins). Collectively, they represent 23 orphan bins of which 4 are comprised of two or more RosCOS.
  3. 3Heterozygous RosCOS in all the genotypes of the bin set resemble bin 4:18 on linkage group 4.