- Research article
- Open Access
Genome-wide identification of Xenopus matrix metalloproteinases: conservation and unique duplications in amphibians
BMC Genomics volume 10, Article number: 81 (2009)
Matrix metalloproteinases (MMPs) are members of the superfamily of Zn2+ dependent extracellular or membrane-bound endopeptidases which have been implicated to play critical roles in vertebrate development and human pathogenesis. A number of MMP genes have been found to be upregulated in some or all organs during frog metamorphosis, suggesting that different MMPs may have different functions in various organs/tissues. The recent advances in EST (expressed sequence tag) sequencing and the completion of the genome of Xenopus (X.) tropicalis prompted us to systematically analyze the existence of MMPs in the Xenopus genome.
We examined X. laevis and X. tropicalis ESTs and genomic sequences for MMPs and obtained likely homologs for 20 out of the 25 MMPs known in higher vertebrates. Four of the five missing MMPs, i.e. MMPs 8, 10, 12 and 27, were all encoded on human Chromosome 11 and the other missing MMP, MMP22 (a chicken MMP), was also absent in human genome. In addition, we identified several novel MMPs which appears to be derived from unique duplications over evolution, are present in the genomes of both Xenopus species.
We identified the homologs of most of the mammalian MMPs in Xenopus and discovered a number of novel MMPs. Our results suggest that MMP genes undergo dynamic changes over evolution. It will be of interest in the future to investigate whether MMP expression and functions during vertebrate development are conserved. The sequence information reported here should facilitate such an endeavor in the near future.
Matrix metalloproteinases (MMPs) are Zn2+ dependent extracellular or membrane-bound proteinases with overlapping substrate specificities [1–6]. They are capable of cleaving proteinaceous components of the extracellular matrix (ECM) as well as non-ECM proteins [2–5, 7, 8], thus affecting cell fate through modifications of cell's microenvironment. MMPs have a similar domain structure that includes a prepeptide for secretion, a propeptide to maintain latency, and a catalytic domain, featured by the signature sequence HEFGHXXH, for substrate cleavage. The catalytic domain binds to a Zn2+ ion through the three-histidine residues within the signature sequence to form the catalytic center [5, 9, 10]. The propeptide contains a highly conserved sequence, PRCGXPD, the so called "cysteine switch", within which the cysteine residue interacts with the catalytic Zn2+ to maintain enzyme latency . Most MMPs are secreted as latent enzymes and processed to the active forms upon the removal of the propeptide domain through various mechanisms. Other MMPs, such as stromelysin 3 (ST3, also known as MMP11), MMP21, MMP23, MMP28, and membrane type MMPs (MT-MMPs) are activated intracellularly through the removal of the propeptide domain by furin, a Golgi enzyme [3, 12, 13].
MMP expression and distribution have long implicated that MMPs play important roles in many physiological processes including embryonic development, angiogenesis, tissue resorption and remodeling, and pathological events such as tumor invasion and arthritis [8, 14–22]. In vitro and cell culture studies have provided strong evidence to show that MMPs can regulate cell fate and behavior by remodeling the ECM. On the other hand, increasing evidences indicate that MMPs are capable of cleaving non-ECM extracellular or membrane-bound proteins, suggesting the existence of multiple pathways for MMPs to regulate cells. Despite the extensive in vitro and cell culture studies, the in vivo functions of MMPs are poorly understood. Surprisingly, with a few exceptions, transgenic overexpression of MMPs and MMP knockouts in mouse have little or weak phenotypes on mouse development [23, 24]. This appears to be at least in part due to the redundancy in MMP expression and function. These findings emphasize the need for further in vivo studies by using different model systems.
Frog metamorphosis offers a unique opportunity to study MMP function during postembryonic development in vertebrates. This process is totally dependent on the presence of thyroid hormone (TH) and mimics the postembryonic period from a few months before to several months after birth in humans [25–27]. During metamorphosis, dramatic tissue-specific remodeling occurs through TH-regulated cell fate changes. These include complete absorption of the gill and the tail, de novo generation of the limbs, and remodeling of most other organs such as the intestine. For example, in the intestine, the larval epithelial cells die through apoptosis and adult epithelial progenitor cells, which may be derived from de-differentiated larval epithelial cells, proliferate and eventually differentiate to form a multiply folded adult epithelium [28–31]. Numerous studies have shown that the metamorphic effects of TH are mediated by thyroid hormone receptors, which control a gene regulation cascade by regulating the transcription of the so-called direct TH-response genes. These direct response genes in turn affect the expression of indirect TH-response genes to eventually regulate cell fate and behavior during metamorphosis. Initial isolation and characterization of TH-response genes revealed that Xenopus (X.) laevis ST3 (MMP11) and collagenase 3 (MMP13), and Rana catesbeiana collagenase 1 (MMP1) are regulated by TH during metamorphosis. Subsequent studies have found that essentially all MMPs analyzed so far are regulated by TH in at least some organs/tissues during metamorphosis [32–48]. Among them, ST3 and MMP9-TH in X. laevis and collagenase 1 in Rana catesbeiana have been shown to be direct response genes with thyroid hormone response elements present in their promoters [43, 49, 50]. Furthermore, in vitro organ culture analysis and in vivo analyses have provided strong evidence for the participation of MMPs in metamorphosis [40, 41, 51–54]. For example, we have demonstrated that ST3 is required for TH-induced ECM remodeling, intestinal larval epithelial apoptosis as well as adult epithelial cell migration in organ cultures and that transgenic overexpression of ST3 alone at premetamorphic stages, e.g., stage 54, can induce larval epithelial apoptosis and ECM remodeling in the intestine in the absence of TH [52, 53]. These functional studies directly proved the function of ST3 as first suggested based on expression analyses. Since all MMPs analyzed so far are regulated by TH during metamorphosis, it is pertinent to ask whether the rest of the MMPs are also regulated by TH and whether different MMPs have different functions during metamorphosis in different organs/tissues.
As an initiative to begin to address these important issues, we have carried out a genome-wide analysis of MMP genes in both X. laevis and X. tropicalis through a bioinformatic approach by making use of the genome sequence information for X. tropicalis and cDNA sequences available for X. laevis and tropicalis genes from the NIH Frog Initiatives Program. We demonstrate that essentially all mammalian MMPs have homologs in Xenopus, although the homologs for some MMPs cannot be assigned with certainty. Furthermore, we have discovered a number of novel MMPs and duplications that are uniquely present in the amphibian genome.
Results and discussion
Bioinformatic search for Xenopus MMPs
Many X. laevis MMPs were previously cloned [32, 33, 39, 41, 43, 44, 46, 47, 55–58]. These cDNA sequences were used to search for other MMPs in the public EST database at the NCBI http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/ and the Gene Index Project in Computational Biology and Function Genomics Laboratory http://compbio.dfci.harvard.edu/tgi/. Putative MMP protein sequences that were derived from the retrieved cDNA sequences were pooled and analyzed on a phylogenetic tree. Closely related entries were compared pair-wise by using MacVector (Accelrys Inc., San Diego, CA) and redundant sequences were removed. The resulting X. laevis MMPs were listed in Table 1 (see Additional file 1 for their nucleotide sequences). Compared to human MMPs, some X. laevis MMPs were missing from the list and some others had highly homologous duplicates, likely due to the pseudotetraploid X. laevis genome. The missing ones could be due to either the absence of the genes in Xenopus genome or incomplete sequence data available. Thus, we also searched and analyzed the cDNA sequences for MMPs in the highly related species, X. tropicalis. We aligned the cDNA sequences of X. laevis or tropicalis MMPs to the JGI X. tropicalis genomic scaffolds http://genome.jgi-psf.org/Xentr4/Xentr4.home.html (Table 1). When needed, we also used human MMP sequences to search the X. tropicalis genome database to ensure a complete search of the genome.
Pair-wise sequence comparison (not shown) and phylogenetic analysis (Fig. 1) allowed us to assign most of the Xenopus MMPs to the corresponding human homologs (except that chicken MMP22 was also used since MMP22 is absent in human ) (where an MMP name had been previously assigned in the databases, we kept the same name for consistency. As we discussed below, some of these MMPs may not be the true homologs of the human MMPs as currently assigned). As shown in table 1, all X. laevis MMPs have a corresponding homolog in X. tropicalis and the homologs are highly conserved with over 80% amino acid sequence identities between X. laevis and X. tropicalis. These include a duplicated gelatinase B (MMP9TH) that is absent in mouse and human genome. As X. laevis is a pseudotetraploid organism, it is not surprising that some MMPs (MMP1, MMP7, MMP13, MMP14, MMP24, MMP28) have duplicate copies in X. laevis but only one in X. tropicalis. Only four X. tropicalis MMPs (MMP N2, MMP N4, MMP N5, and MMP N6, see below for more on these MMPs) have no available homologs in X. laevis yet, likely due to incomplete cDNA sequence information available for X. laevis.
Xenopus MMPs with known human homologs
Phylogenetic (Fig. 1) and pair-wise sequence (table 2) analyses suggest that the following human MMPs have true homologs in Xenopus genome: MMP2, MMP9, MMP11, MMP13, MMP14, MMP15, MMP16, MMP17, MMP19, MMP20, MMP21, MMP23, MMP24, MMP25, MMP28. The homologous human and X. tropicalis MMPs cluster together in the phylogenetic tree (Fig. 1) and share highest sequence identities with each other than with any other MMPs (one exception to this is X. tropicalis MMP25, which shares similar homologies with human MMP17 and MMP25. Since the structurally related X. tropicalis MMP17 share 70% homology with the human MMP17 but only 47% homology with the human MMP25, we assigned this MMP as X. tropicalis MMP25) (table 2). In addition, the homologous MMPs have similar lengths and domain organizations (data not shown).
Likely Xenopus homologs of human MMPs
The homologs of the rest of human MMPs could not be easily identified based on sequence comparison and phylogenetic analysis. These MMPs may have corresponding homologs in Xenopus but their sequences have diverged significantly that it is difficult to match the human and Xenopus counterparts. For these MMPs, we kept the putative names for any Xenopus MMPs with previously assigned names in the public databases or assigned the names as described below.
There were two entries for X. laevis MMP1 (GenBank accession # BC054233 and BC084836), encoding two closely related MMPs of 466 amino acids (aa) that are 90% identical (data not shown). Alignment of these X. laevis MMP1s (MMP1A and MMP1B) to the X. tropicalis genomic scaffolds showed significant homology at three different loci on the Scaffold_119 (Note that the X. tropicalis genomic sequence is not complete and the individual sequences are assembled into scaffolds instead of individual chromosomes). The putative cDNA sequences were derived from these loci and used to deduce the protein sequences of three related MMPs. Among these three putative MMP genes, the best-matched one has 87% identities with X. laevis MMP1s and has the same length; it was therefore named as X. tropicalis MMP1 (Table 1). The other two were tentatively named as X. tropicalis MMP N4 and N5 (Table 1).
Phylogenetic analysis revealed that X. tropicalis MMP1, MMP N4 and N5 cluster together with MMP N4 and N5 more closely related to each other than to MMP1 (Fig. 1). These MMPs are related to several human MMP subfamilies including collagenases (MMP1 and 8) and stromelysins (MMP3 and 10), etc. The MMP that is most closely related to these three MMPs is X. tropicalis MMP18, a homolog of X. laevis MMP18 (Table 1). X. laevis MMP18 is a known collagenase , suggesting that these three MMPs are collagenases. Apart from the typical MMP domains (i.e., the signal peptide, the conserved zinc binding motif characteristic of MMPs, and the conserved cysteine-switch domain within the propeptide), X. tropicalis MMP1, as well as X. laevis MMP1A and 1B, contains a 16 aa proline-rich motif after the catalytic domain that distinguishes collagenases from stromelysins, although the X. tropicalis MMP N4 and N5 have deletions within the region (X. tropicalis MMP N5 lacks the entire hinge domain but has the intact catalytic and hemopexin domain, a characteristic similar to that of the MMP21s) (Fig. 2). These MMPs share less than 60% identity with X. laevis collagenases MMP13 and MMP18. In addition, they are also three amino acids shorter than the human MMP1 at the C- terminus (Fig. 2), just like the X. laevis MMP13 and MMP18. Taken together, these MMP genes are likely X. tropicalis collagenases, but it is possible that the X. tropicalis MMP1 is not the true homolog of human MMP1, especially considering that the MMP1 from another amphibian species, Rana catesbeiana, is much more homologous to human MMP1, although much shorter, compared to Xenopus MMP1 (Fig. 1) .
MMP3 and MMP10, also referred to as stromelysin 1 and 2, respectively, are MMPs that have quite broad substrate specificities and were originally described as proteoglycanases [59–62]. Neither Xenopus MMP3 nor MMP10 has been characterized. A putative MMP deduced from a cDNA entry for each Xenopus species (GenBank accession number: BC077966 of X. laevis clone and NM_001030331 of X. tropicalis clone) structurally resembles MMP3 and MMP10 (Fig. 3). Similar to human MMP3 and MMP10, the Xenopus MMP has an insertion (8 aa for the X. tropicalis MMP and 14 aa for the X. laevis MMP) in the 16 aa proline-rich motif after the catalytic domain whose integrity is important for collagenase activity (Fig. 3). This suggests that the Xenopus MMP is likely a stromelysin. The two Xenopus homologs share 84% identity, although the X. laevis one is 45 aa shorter than X. tropicalis MMP3 at the N-terminus, likely due to incomplete 5'-end cDNA sequence. Thus, the Xenopus MMPs are homologs of each other and are tentatively named as MMP3 since they are slightly more similar to human MMP3 than MMP10 (Table 2).
MMP23 is characterized by the presence of a furin activation site, a type II transmembrane domain at the N-terminus, and a unique truncated C-terminal domain unrelated to the hemopexin domain found in other MMPs, and it lacks a typical prepeptide [63–66]. There are two reported MMP23, MMP23A and MMP23B, in human that are encoded by two genes, likely due to a very recent, partial duplication at Chromosome 1p36.3 . Human MMP23A and B are identical in amino acid sequences and thus both are referred to as MMP23 here. Two overlapping EST entries (CD302225 and CD302813) encode a putative X. laevis MMP23 of 381aa. Sequence search of the X. tropicalis genome identified a putative X. tropicalis MMP23 on Scaffold_414. There was no EST entry representing the X. tropicalis MMP23. However, two X. tropicalis EST entries (CX344815 and CX344816) composed of cDNA sequences that together encode another putative MMP related to MMP23 (tentatively named as MMP N6). These cDNA sequences aligned on to X. tropicalis genomic Scaffold_132. X. tropicalis MMP23 and MMP N6 differ from each other at both the cDNA and protein sequence levels, unlike the two human MMP23 genes (note that there is only one MMP23 in the mouse genome). Sequence comparison showed that X. laevis MMP23, X. tropicalis MMP23 and MMP N6 have the same features of human MMP23, although there is a sequence gap in X. tropicalis MMP23 (Fig. 4), possibly due to incomplete genomic sequence information (see Additional file 1). The Xenopus MMP 23, MMP N6, and human MMP23 cluster together and are away from all other MMPs (Fig. 1) with the MMP23 sharing 60% identity between Xenopus and human, similar to other homologous MMPs (table 2).
MMP21 was first cloned in X. laevis . It has since been found to be present in other vertebrates including human. The common features of this MMP across different species, in addition to those characteristics of MMPs, are a putative furin cleavage site between the propetide and the catalytic domain, a relative long insertion (20~44 aa) between the PRCGXPD cysteine switch motif and the furin cleavage site (RXKR), and a unique cysteine residue in the catalytic domain (Fig. 5). The putative X. tropicalis MMP21 composes of 604 aa and shares 92% and 59% identities with the X. laevis and human MMP21, respectively (Tables 1 and 2).
Interestingly, sequence search also revealed a cDNA entry (TC84199), composed of X. tropicalis ESTs DR836290 and CX386748, that encodes a putative MMP of 524 aa. This MMP is highly homologous to X. tropicalis MMP21 and is tentatively named as X. tropicalis MMP N3 (Fig. 5). In addition, 4 highly homologous X. laevis ESTs, BG234242, BU905338, CB558404, and CF547511, were also found to constitute a putative cDNA sequence encoding a homolog of X. tropicalis MMP N3 (see Additional file 1, Table 1, and Fig. 5). The X. tropicalis MMP N3 is located on the X. tropicalis genomic Scaffold_508, from which the rest 5'-end cDNA sequence was predicted. The putative X. tropicalis MMP N3 encodes a predicted protein of 627 aa. The N-terminal sequence of X. laevis MMP N3 is still missing in the databases. Sequence comparison clearly showed that MMP N3 is highly homologous to MMP21 and is likely derived from a gene duplication event (Fig. 5). Furthermore, phylogenetic analysis indicated that Xenopus MMP21 clusters with human MMP21 and is closely related to MMP N3.
MMP7 and 26
MMP7 and MMP26 are the smallest MMPs known and have no hemopexin domain at their C-terminus [62, 68–70]. X. laevis MMP7 was previously reported (GenBank accession # AF573380) and found to be expressed specifically in tissue resident macrophages . Bioinformatic search revealed a closely related X. laevis cDNA clone (GenBank accession # BC056040) encoding a protein of 259 aa vs. 252 aa for X. laevis MMP7. These two X. laevis MMPs shared 85% identities in amino acid sequences and 82% identities in nucleotide sequences, and therefore, are likely duplicated MMP7 genes in the pseudotetraploid genome. We designated them as MMP7A (GenBank accession # AF573380) and 7B (GenBank accession # BC056040), respectively. Both X. laevis MMP7A and 7B aligned to X. tropicalis Scaffold_119 at the location that encodes a X. tropicalis cDNA (GenBank accession # NM_001005043). Thus, this X. tropicalis c DNA represents X. tropicalis MMP7. X. tropicalis MMP7 is 259 aa in length and highly homologous to X. laevis MMP7A/B (Tables 1 and Fig. 6).
In addition to the MMP7 genes, the X. laevis cDNA clone MGC69070 (GenBank accession # BC056080) and X. tropicalis cDNA clone MGC108008 (GenBank accession # NM_001032335) also encode a small MMP each (258 aa and 261 aa, respectively). Like MMP7, both proteins lacked the hemopexin domain (Fig. 6). They share 88% identities with each other and the X. tropicalis gene is also located on X. tropicalis genomic Scaffold_119. These two proteins are closely related to X. laevis MMP7A/B and X. tropicalis MMP7 on the phylogenetic tree and thus are tentatively named as X. laevis MMP26 and X. tropicalis MMP26, respectively. (It should be pointed out that it is difficult to assign with certainty which of the Xenopus gene is the homolog of human MMP7 and which is that of human MMP26. For consistency, we kept Xenopus MMP7 for the previously published sequence ).
Surprisingly, an additional X. tropicalis clone (IMAGE7719439, EST# CX982585 and CX982586) was also found to encode a similar MMP lacking the hemopexin domain. The cDNA sequence had two in-frame stop codons after the 3'-end of the coding sequence (see Additional file 1) and another independent EST sequence (EST #CX979196) overlapped with this region with 100% identity (data not shown). Thus, this gene represents a novel MMP that is structurally similar to MMP7 and MMP26. It is tentatively named as X. tropicalis MMP N2 (Fig. 6). X tropicalis MMP N2 is also located on X. tropicalis Scaffold_119 in between MMP7 and MMP26, suggesting that it was derived from a gene duplication event.
Novel Xenopus MMPs
Sequence analysis revealed the existence of an alternatively spliced form of X. laevis gelatinase A (MMP2) (MMP2asv, Fig. 7). This alternatively spliced MMP2 transcript encodes a MMP that lacks most of the catalytic domain, including the zinc binding motif, and part of the C-terminal hemopexin domain. To date, no such spliced form of MMP2 has been reported for other vertebrate species, including X. tropicalis.
In addition, there are two MMPs in both X. laevis and X. tropicalis that are highly homologous to MMP9 or gelatinase B, MMP9 and MMP9TH, respectively  (Tables 1 and 2), and both cluster with human MMP9 in the phylogenetic tree (Fig. 1). Xenopus MMP9 and MMP9TH have all the features characteristic of a gelatinase (data not shown). Since only one MMP9 genes have been found in other vertebrates, MMP9TH and MMP9 represent a unique duplication in Xenopus.
The assembly of a group of overlapping X. laevis ESTs (EST # BE509380, BJ032306, BG813136, EB469763, EC276067, BX852582, BJ047339, and BG578455) led to a cDNA (see Additional file 1) encoding a protein of 562 aa that lacks the N-terminus and shares low levels of homologies with others MMPs but has all the characteristics of an MMP (Fig. 8 and data not shown). Similarly, the homologous X. tropicalis ESTs could be assembled into a cDNA encoding a full length MMP of 573 aa that is 90% homologous to the X. laevis counterpart (Fig. 8 and table 1). This X. tropicalis MMP has less than 40% homology to any of human MMPs and represents a novel frog MMP tentatively named as X. tropicalis MMP N1 (Fig. 8 and Table 2). Its counterpart in X. laevis is therefore named as X. laevis MMP N1 (Fig. 8 and Table 1).
While the Xenopus MMP N1 has all the domains typical of an MMP, there are some differences distinguishing it from other MMPs. First, both X. laevis and X. tropicalis MMP N1 had a "cysteine-switch" made of "PRCGKHE" instead of the conserved "PRCGXXD" sequence and a deletion of two amino acid residues around the predicted cleavage site for the mature MMPs (Fig. 8). Second, these two MMPs are most similar to collagenases and stromelysins in their domain organization but have a 16 aa insertion in the 16 aa collagen-binding motif of collagenases at the exact position where an insertion is found for stromelysins (Fig. 8). Finally, there are a number of insertions throughout Xenopus MMP N1 compared to human collagenases and stromelysins (Fig. 8). These unique features suggest that Xenopus MMP N1 is a novel MMP distinct from other known MMPs.
As described above, there are three X. tropicalis MMPs homologous to human MMP7 and MMP26. Sequence analyses allowed us to assign two of them as X. tropicalis MMP7 and MMP26, respectively. The third one thus represents a novel gene of this subfamily (Figs. 1 and 6). Interestingly, all three X. tropicalis MMP genes are located consecutively on a single chromosome with MMPN2 in between MMP7 and MMP26 (Fig. 9), suggesting that a duplication event in amphibians led to the extra MMP in the subfamily.
MMP N3 is one of the two genes in both X. laevis and X. tropicalis that have similar levels of homology to human MMP21 (Figs. 1 and 5). Although MMP N3 and MMP21 are located in different scaffolds in X. tropicalis genomic sequence, it is possible that they are located adjacent to each other in a chromosome as the genomic sequence annotation is incomplete in X. tropicalis. Thus, these two genes might have derived from a unique gene duplication event in amphibians.
Based on sequence features and its enzymatic activity, X. laevis MMP18 was proposed to be a novel collagenase . Indeed, potential homologs of mammalian collagenases MMP1 and MMP13 have been reported for Xenopus. Interestingly, while the amphibian MMP13 and human MMP13 are 67% identical, the corresponding MMP1s are only 51% identical (Table 2), similar to the levels of homology that MMP18 has with human collagenases (MMP1, 8, and 13). Furthermore, human MMP1 and MMP8 have 3 extra amino acids (RKN) at the C-terminus that are lacking in Xenopus MMP1 and MMP18 as well as the putative, novel Xenopus collagenases (MMP N4 and N5) (Fig. 2). In addition, a most likely homolog of human MMP1 has been reported for another amphibian species, Rana catesbeiana . The Rana MMP1 is much shorter but much more homologous to human MMP1 (about 80%), compared to these Xenopus collagenases (data not shown and ). Furthermore, unlike the Xenopus MMP1, the Rana MMP1 clusters with human MMP1 on the phylogenetic tree (Fig. 1). Thus, it is possible that MMP1 has diverged extensively between amphibians and mammals, leading to a very different size in Rana or its loss in Xenopus. On the other hand, all known and putative collagenases in Xenopus are located consecutively in a single chromosome in the order of MMP1, MMP N4, MMP N5, MMP18, and MMP13 (Fig. 9). This suggests that multiple duplication events might be responsible for the generation of these MMPs. It is interesting to note that human MMP 1, 3, 7, 8, 10, 12, 13, 20, 26 and 27 are all encoded by Chromosome 11 (Fig. 9). With the exception of four MMPs (MMP8, 10, 12 and 27) that have no apparent homologs in X. tropicalis, the other six MMPs found on human Chromosome 11 are clustered on the Scaffold_119 together with four frog-specific ones (MMP18, MMP N2, N4, and N5) in X. tropicalis (Fig. 9). Thus, X. tropicalis Scaffold_119 appears to contain a large region syntenic to human Chromosome 11 . On the other hand, with the exception of MMP13 and MMP20, it is difficult to determine which MMP in this cluster of 10 X. tropicalis MMPs is the homolog of an MMP in the human cluster based on sequence homology and syntenic analyses. X. tropicalis MMP13 and MMP20 share high degrees of homology (about 70%) with their human counterparts, supporting that they are true homologs of the human MMPs. Consistently, phylogenetic analysis of X. tropicalis and human MMPs showed that these two MMPs evolved earlier than the other MMPs in the cluster (see Additional file 2). In addition, the drastic differences in the distances between MMP7 and MMP26 on the chromosome (100 mega bp in human and 100 kb in Xenopus) (Fig. 9) and on the phylogenetic trees (Supplemental Fig. 2), suggest that this duplication occurred after the separation of amphibians from mammals. The other six MMPs in the cluster appear to have diverged rapidly during evolution and/or evolved through duplications and/or losses independently in amphibians and mammals.
Through a bioinformatic approach, we have identified Xenopus homologs for most human MMPs. By comparing the MMPs in the two highly related species, X. tropicalis and X. laevis, we have been able to discover several unique duplications of MMPs genes in amphibians that are absent in mammals. On the other hand, several human MMPs have no apparent homologs in Xenopus and were possibly evolved de novo in mammals. Among the likely duplicated genes, genes in the following two pairs, MMP9 and MMP9TH, MMP21 and MMP N3, MMP 7 (or MMP26) and MMP N2, are more homologous to each other than to their human homologs (Fig. 1), suggesting that the duplications occurred after the separation of amphibians from mammals. On the other hand, MMP23 clusters closer to its human homolog than to the putative duplicate MMP N6 (Fig. 1), suggesting the possibility that MMP23 might have duplicated before the separation of amphibians and mammals and one copy was lost subsequently in mammals. Duplications and loss in MMP genes are also evident when comparing the largest MMP cluster located on human Chromosome 11 with the MMP cluster on X. tropicalis Scaffold_119, where a number of novel MMPs were found and several MMPs were lost in X. tropicalis. Our findings thus demonstrate a dynamic process for MMP gene evolution. It will be of interest in the future to investigate whether MMP expression and function are conserved during vertebrate development. The sequence information reported here and the advantage of the amphibian metamorphosis for functional studies in vivo should facilitate such an endeavor in the near future.
We first searched the public EST database at the National Center for Biotechnology Information (NCBI, http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/) and the Gene Index Project in Computational Biology and Function Genomics Laboratory http://compbio.dfci.harvard.edu/tgi/ with known Xenopus MMP genes for other possible MMP sequences based on sequence similarities. The identities of putative Xenopus MMPs were tentatively determined by building a phylogenetic tree with human MMPs. This was done through Multiple Sequence Alignment by CLUSTALW http://align.genome.jp/. Human MMPs used were: MMP1 (NP_002412), MMP2 (NP_004521), MMP3 (NP_002413), MMP7 (NP_002414), MMP8 (NP_002415), MMP9 (NP_004985), MMP10 (NP_002416), MMP11 (NP_005931), MMP12 (NP_002417), MMP13 (NP_002418), MMP14 (NP_004986), MMP15 (NP_002419), MMP16 (NP_005932), MMP17 (NP_057239), MMP19 (NP_002420), MMP20 (NP_004762), MMP21 (NP_671724), MMP22 (NP_990331), MMP23 (NP_008914), MMP24 (NP_006681), MMP25 (NP_071913), MMP26 (NP_068573), MMP27 (NP_071405), and MMP28 (NP_077278). The X. laevis MMPs were: MMP1A (BC054233), MMP1B (BC084836), MMP2 (AY037943), MMP3 (BC077966), MMP7A (AY573380), MMP7B (BC056040), MMP9 (AF072455), MMP9TH (AB288054), MMP11 (Z27093), MMP13 (L49412), MMP13A (U41824), MMP14A (AY633953), MMP14B (BC077870), MMP15 (AY573378), MMP16 (AY310397), MMP17 (CK806816), MMP18 (L76275), MMP19 (BX847184), MMP20 (DQ885892), MMP21 (U82541), MMP23 (CD302225 &CD302813), MMP24A (CA791076 &EB480268), MMP24B (EB483310), MMP25 (BC078136), MMP26 (BC056080), MMP28A (EF187277), MMP28B (BC061659), MMP N1 (Assembly of BJ032306, BE509380, EC276067, BX852582, BJ047339 and BG578455) and MMP N3 (Assembly of BG234242, BU905338, CB558404, and CF547511) (See Supplemental Fig. 1). Pair-wise comparison of protein sequences was conducted by using MacVector (Accelrys Inc., San Diego, CA) to further confirm the identity assignment. X. laevis or tropicalis cDNA sequences were used to do BLAST search against the X. tropicalis genome assembly 4.1 http://genome.jgi-psf.org/Xentr4/Xentr4.home.html to determine the corresponding gene structures and predict cDNA sequences if necessary. The X. tropicalis MMPs thus obtained from the GenBank database were: MMP2 (NM_001015789), MMP3 (NM_001030331), MMP7 (NM_001005043), MMP9 (NM_001006842), MMP14 (NM_001030388), MMP15 (NM_001015921), MMP16 (NM_001015992), MMP17 (NM_001102999), MMP18 (NM_001030330), MMP19 (BC153750), MMP25 (CU075461), MMP26 (NM_001032335), MMP N1 (BC155487, DN028798, DN034177 and DN076875), MMP N2 (CX982585, CX982586 and CX979196), MMP N6 (CX344816 and CX344815). Other X. tropicalis MMP sequences were derived from predicted exons of the genomic sequences (see Additional file 1 and Table 1 for their sequences and locations in the genome).
expressed sequence tags
Overall CM: Molecular determinants of metalloproteinase substrate specificity. Molecular Biotechnology. 2002, 22: 51-86.
Alexander CM, Werb Z: Extracellular matrix degradation. Cell Biology of Extracellular Matrix. Edited by: Hay ED. 1991, New York.: Plenum Press, 255-302. 2
McCawley LJ, Matrisian LM: Matrix metalloproteinases: they're not just for matrix anymore!. Current Opinion in Cell Biology. 2001, 13: 534-540.
Parks WC, Mecham RP: Matrix metalloproteinases. 1998, New York: Academic Press
Nagase H: Cell surface activation of progelatinase A (proMMP-2) and cell migration. Cell Res. 1998, 8 (3): 179-186.
Pei D: Leukolysin/MMP25/MT6-MMP: a novel matrix metalloproteinase specifically expressed in the leukocyte lineage. Cell Res. 1999, 9 (4): 291-303.
Mott JD, Werb Z: Regulation of matrix biology by matrix metalloproteinases. Current Opinion in Cell Biology. 2004, 16: 558-564.
Uria JA, Werb Z: Matrix metalloproteinases and their expression in mammary gland. Cell Res. 1998, 8 (3): 187-194.
Murphy G, Stanton H, Cowell S, Butler G, Knauper V, Atkinson S, Gavrilovic J: Mechanisms for pro matrix metalloproteinase activation. Apmis. 1999, 107 (1): 38-44.
Nagase J, Suzuki K, Morodomi T, Englhild JJ, Salvesen G: Activation Mechanisms of the Precursors of Matrix Metalloproteinases 1,2, and 3. Matrix Suppl. 1992, 1: 237-244.
van Wart HE, Birkedal-Hansen H: The cysteine switch: A principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family. Proc Natl Acad Sci USA. 1990, 87: 5578-5582.
Pei D, Weiss SJ: Furin-dependent intracellular activation of the human stromelysin-3 zymogen. Nature. 1995, 375 (6528): 244-247.
Sato H, Seiki M: Membrane-Type Matrix Metallproteinases (MT-MMPs) in Tumor Metastasis. J Biochem. 1996, 119: 209-215.
Basset P, Bellocq JP, Lefebvre O, Noel A, Chenard MP, Wolf C, Anglard P, Rio MC: Stromelysin-3: a paradigm for stroma-derived factors implicated in carcinoma progression. Crit Rev Oncol Hematol. 1997, 26 (1): 43-53.
Lochter A, Bissell MJ: An odyssey from breast to bone: multi-step control of mammary metastases and osteolysis by matrix metalloproteinases. APMIS. 1999, 107 (1): 128-136.
MacDaougall JR, Matrisian LM: Contributions of tumor and stromal matrix metalloproteinases to tumor progression, invasion and metastasis. Cancer Metastasis Rev. 1995, 14: 351-362.
Tryggvason K, Hoyhtya M, Salo T: Proteolytic degradation of extracellular matrix in tumor invasion. Biochim Biophys Acta. 1987, 907 (3): 191-217.
Matrisian LM, Hogan BL: Growth factor-regulated proteases and extracellular matrix remodeling during mammalian development. Curr Top Dev Biol. 1990, 24: 219-259.
Shi Y-B, Fu L, Hasebe T, Ishizuya-Oka A: Regulation of ECM remodeling and cell fate determination by matrix metalloproteinase stromelysin-3 during thyroid hormone-dependent postembryonic development. Pharmacology & Therapeutics. 2007, 116: 391-400.
Fu L, Hasebe T, Ishizuya-Oka A, Shi Y-B: Roles of matrix metalloproteinases and ECM remodeling during thyroid hormone-dependent intestinal metamorphosis in Xenopus laevis. Organogenesis. 2007, 3: 14-19.
Sarras MPJ, Yan L, Leontovich A, Zhang JS: Structure, expression, and developmental function of early divergent forms of metalloproteinases in hydra. Cell Research. 2002, 12: 163-176.
Sang QX: Complex role of matrix metalloproteinases in angiogenesis. Cell Res. 1998, 8 (3): 171-177.
Shapiro SD: Matrix metalloproteinase degradation of extracellular matrix: biological consequences. Curr Opin Cell Biol. 1998, 10 (5): 602-608.
Page-McCaw A, Ewald AJ, Werb Z: Matrix metalloproteinases and the regulation of tissue remodelling. Nat Rev Mol Cell Biol. 2007, 8 (3): 221-233.
Yen PM: Physiological and molecular basis of thyroid hormone action. Physiol Rev. 2001, 81 (3): 1097-1142.
Tata JR: Gene expression during metamorphosis: an ideal model for post-embryonic development. Bioessays. 1993, 15 (4): 239-248.
Shi Y-B: Amphibian Metamorphosis: From morphology to molecular biology. 1999, New York: John Wiley & Sons, Inc
Amano T, Noro N, Kawabata H, Kobayashi Y, Yoshizato K: Metamorphosis-associated and region-specific expression of calbindin gene in the posterior intestinal epithelium of Xenopus laevis larva. Dev Growth Differ. 1998, 40: 177-188.
Schreiber AM, Cai L, Brown DD: Remodeling of the intestine during metamorphosis of Xenopus laevis. Proc Natl Acad Sci USA. 2005, 102 (10): 3720-3725.
Shi Y-B, Ishizuya-Oka A: Thyroid hormone regulation of apoptotic tissue remodeling: Implications from molecular analysis of amphibian metamorphosis. Prog Nucleic Acid Res Mol Biol. 2001, 65: 53-100.
Shi Y-B, Ishizuya-Oka A: Biphasic intestinal development in amphibians: Embryogensis and remodeling during metamorphosis. Curr Top Dev Biol. 1996, 32: 205-235.
Shi Y-B, Brown DD: The earliest changes in gene expression in tadpole intestine induced by thyroid hormone. J Biol Chem. 1993, 268: 20312-20317.
Patterton D, Hayes WP, Shi YB: Transcriptional activation of the matrix metalloproteinase gene stromelysin-3 coincides with thyroid hormone-induced cell death during frog metamorphosis. Dev Biol. 1995, 167 (1): 252-262.
Ishizuya-Oka A, Ueda S, Shi Y-B: Transient expression of stromelysin-3 mRNA in the amphibian small intestine during metamorphosis. Cell Tissue Res. 1996, 283 (2): 325-329.
Damjanovski S, Ishizuya-Oka A, Shi YB: Spatial and temporal regulation of collagenases-3, -4, and stromelysin – 3 implicates distinct functions in apoptosis and tissue remodeling during frog metamorphosis. Cell Res. 1999, 9 (2): 91-105.
Berry DL, Schwartzman RA, Brown DD: The expression pattern of thyroid hormone response genes in the tadpole tail identifies multiple resorption programs. Dev Biol. 1998, 203: 12-23.
Berry DL, Rose CS, Remo BF, Brown DD: The expression pattern of thyroid hormone response genes in remodeling tadpole tissues defines distinct growth and resorption gene expression programs. Dev Biol. 1998, 203: 24-35.
Shi YB, Fu L, Hsia SC, Tomita A, Buchholz D: Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis. Cell Res. 2001, 11 (4): 245-252.
Wang Z, Brown DD: Thyroid hormone-induced gene expression program for amphibian tail resorption. J Biol Chem. 1993, 268: 16270-16278.
Oofusa K, Yomori S, Yoshizato K: Regionally and hormonally regulated expression of genes of collagen and collagenase in the anuran larval skin. Int J Dev Biol. 1994, 38 (2): 345-350.
Jung J-C, Leco KJ, Edwards DR, Fini ME: Matrix metalloproteinase mediate the dismantling of mesenchymal structures in the tadpole tail during thyroid hormone-induced tail resorption. Dev Dyn. 2002, 223: 402-413.
Stolow MA, Bauzon DD, Li J, Sedgwick T, Liang VC, Sang QA, Shi YB: Identification and characterization of a novel collagenase in Xenopus laevis: possible roles during frog development. Mol Biol Cell. 1996, 7 (10): 1471-1483.
Fujimoto K, Nakajima K, Yaoita Y: One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis. Dev Growth Differ. 2006, 48 (4): 223-241.
Fujimoto K, Nakajima K, Yaoita Y: Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis. Dev Growth Differ. 2007, 49 (2): 131-143.
Hasebe T, Kajita M, Fujimoto K, Yaoita Y, Ishizuya-Oka A: Expression profiles of the duplicated matrix metalloproteinase-9 genes suggest their different roles in apoptosis of larval intestinal epithelial cells during Xenopus laevis metamorphosis. Dev Dyn. 2007, 236 (8): 2338-2345.
Hasebe T, Hartman R, Matsuda H, Shi YB: Spatial and temporal expression profiles suggest the involvement of gelatinase A and membrane type 1 matrix metalloproteinase in amphibian metamorphosis. Cell Tissue Res. 2006, 324: 105-116.
Hammoud L, Walsh LA, Damjanovski S: Cloning and developmental characterization of Xenopus laevis membrane type-3 matrix metalloproteinase (MT3-MMP). Biochem Cell Biol. 2006, 84: 167-177.
Walsh LA, Carere DA, Cooper CA, Damjanovski S: Membrane type-1 matrix metalloproteinases and tissue inhibitor of metalloproteinases-2 RNA levels mimic each other during Xenopus laevis metamorphosis. PLoS ONE. 2007, 2 (10): e1000-
Fu L, Tomita A, Wang H, Buchholz DR, Shi Y-B: Transcriptional regulation of the Xenopus laevis stromelysin-3 gene by thyroid hormone is mediated by a DNA element in the first intron. J Biol Chem. 2006, 281: 16870-16878.
Oofusa K, Yoshizato K: Thyroid hormone-dependent expression of bullfrog tadpole collagenase gene. Roux's Arch Dev Biol. 1996, 205: 241-251.
Damjanovski S, Amano T, Li Q, Pei D, Shi Y-B: Overexpression of Matrix Metalloproteinases Leads to Lethality in Transgenic Xenopus Laevis: Implications for Tissue-Dependent Functions of Matrix Metalloproteinases during Late Embryonic development. Dev Dyn. 2001, 221 (1): 37-47.
Ishizuya-Oka A, Li Q, Amano T, Damjanovski S, Ueda S, Shi Y-B: Requirement for matrix metalloproteinase stromelysin-3 in cell migration and apoptosis during tissue remodeling in Xenopus laevis. J Cell Biol. 2000, 150 (5): 1177-1188.
Fu L, Ishizuya-Oka A, Buchholz DR, Amano T, Matsuda H, Shi YB: A causative role of stromelysin-3 in extracellular matrix remodeling and epithelial apoptosis during intestinal metamorphosis in Xenopus laevis. J Biol Chem. 2005, 280 (30): 27856-27865.
Hasebe T, Fu L, Amano T, Shi Y-B: Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development. Mechanisms of Development. 2007, 124: 11-22.
Shintani S, Kobata M, Kamakura N, Toyosawaa S, Ooshima T: Identification and characterization of matrix metalloproteinase-20 (MMP20; enamelysin) genes in reptile and amphibian. Gene. 2007, 392: 89-97.
Harrison M, Abu-Elmagd M, Grocott T, Yates C, Gavrilovic J, Wheeler GN: Matrix metalloproteinase genes in Xenopus development. Dev Dyn. 2004, 231 (1): 214-220.
Yang M, Kurkinen M: Cloning and characterization of a novel matrix metalloproteinase (MMP), CMMP, from chicken embryo fibroblasts. J Biol Chem. 1998, 273: 17893-17900.
Tomlinson ML, Garcia-Morales C, Abu-Elmagd M, Wheeler GN: Three matrix metalloproteinases are required in vivo for macrophage migration during embryonic development. Mech Dev. 2008, 125: 1059-1070.
Saus J, Quinones S, Otani Y, Nagase H, Harris ED, Kurkinen M: The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin. J Biol Chem. 1988, 263 (14): 6742-6745.
Whitham SE, Murphy G, Angel P, Rahmsdorf H-J, Smith BJ, Lysons A, Harris TJR, Reynolds JJ, Herrlich P, Docherty AJP: Comparison of human stromelysin and collagenase by cloning and sequence analysis. Biochem J. 1986, 240 (3): 913-916.
Murphy GJP, Murphy G, Reynolds JJ: The origin of matrix metalloproteinases and their familial relations. FEBS Letters. 1991, 289: 4-7.
Muller D, Quantin B, Gesnel MC, Millon-Collard R, Abecassis J, Breathnach R: The collagenase gene family in humans consists of at least four members. Biochem J. 1988, 253 (1): 187-192.
Pei D: CA-MMP: a matrix metalloproteinase with a novel cysteine array, but without the classic cysteine switch. FEBS Letters. 1999, 457: 262-270.
Velasco G, Pendás AM, Fueyo A, Knäuper V, Murphy G, López-Otín C: Cloning and characterization of human MMP23, a new matrix metalloproteinase predomainantly expressed in reproductive tissues ana lacking conserved domains In other family members. J Biol Chem. 1999, 274: 4570-4576.
Gururajan R, Grenet J, Lahti JM, Kidd VJ: Isolation and characterization of two novel metalloproteinase genes linked to the Cdc2L locus on human chromosome 1p36.3. Genomics. 1998, 52: 101-106.
Ohnishi J, Onishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, et al: Cloning and characterization of a rat ortholog of MMP23 (Matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development. Mol Endocrinol. 2001, 15: 747-764.
Yang M, Murray MT, Kurkinen M: A novel matrix metalloproteinase gene (XMMP) encoding vitronectin-like motif is transiently expressed in Xenopus laevis early embryo development. J Biol Chem. 1997, 272: 13527-13533.
Park HI, Ni J, Gerkema FE, Liu D, Belozerov VE, Sang Q-XA: Identification and characterization of human endometase (Matrix Metalloproteinase-26) from endometrial tumor. J Biol Chem. 2000, 275: 20540-20544.
de Coignac AB, Elson G, Delneste Y, Magistrelli G, Jeannin P, Aubry J-P, Berthier O, Schmitt D, Bonnefoy J-Y, Gauchat J-F: Cloning of MMP-26: A novel matrilysin-like proteinase. Eur J Biochem. 2000, 267: 3323-3329.
Marchenko GN, Ratnikov BI, Rozanov DV, Godzik A, Deryugina EI, Strongin AY: Characterization of matrix metalloproteinase-26, a novel metalloproteinase widely expressed in cancer cells of epithelial origin. Biochem J. 2001, 356: 705-718.
Loots GG, Ovcharenko I: ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes. Bioinformatics. 2007, 23: 122-124.
This research was supported by the Intramural Research Program of NICHD, NIH.
LF collected sequence information, performed bioinformatic analysis and wrote the first draft; BD and SM performed bioinformatic analysis and edited the manuscript; and YS supervised the research and finalized the paper. All the authors critically revised and approved the final version of the paper.
Electronic supplementary material
Additional file 1: The nucleotide sequences of the X. tropicalis and X. laevis MMPs. The data presented all the all the nucleotide sequences of the X. tropicalis and X. laevis MMPs that were used for deducing Xenopus MMPs in the study. GenBank accession numbers or the scaffold of the X. tropicalis genome on which the Xenopus MMP locates were included if applicable. (DOC 252 KB)
Additional file 2: Phylogenetic trees of X. tropicalis and human MMPs. X. tropicalis MMPs along with Rana catesbeiana MMP1 (RcMMP1) or human MMPs along with chicken MMP22 (CMMP22) were analyzed using the multiple sequence alignment program CLUSTALW to generate the corresponding phylogenetic trees with defined ancestral nodes marked by purple square. The MMPs located on human Chromosome 11 and those located on X. tropicalis Scaffold_119 are in red. (TIFF 340 KB)
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Fu, L., Das, B., Mathew, S. et al. Genome-wide identification of Xenopus matrix metalloproteinases: conservation and unique duplications in amphibians. BMC Genomics 10, 81 (2009). https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2164-10-81
- Rana Catesbeiana
- True Homolog
- Tropicalis Genome
- Furin Cleavage Site
- Hemopexin Domain