Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levels

Table 2 The regulation of transcripts and proteins of the polyamine pathway in three distinct inhibition experiments.

Annotation	PlasmoDB ID	Cyclohexylamine (PfSpdSyn inhibition) (This work)		DFMO/MDL73811 (PfAdoMetDC/ODC inhibition) [9]		DFMO (ODC inhibition) [28]
		Transcript	Protein	Transcript	Protein	Transcript
Ornithine aminotransferase	PFF0435w	≈	↓	↑	↑	↑
S-adenosylmethionine decarboxylase-ornithine decarboxylase (PfAdoMetDC/ODC)	PF10_0322	≈	nd	↓	nd	nd
Hypoxanthine phosphoribosyltransferase	PF10_0121	≈	nd	≈	nd	↑
Uridine phosphorylase, putative	PFE0660c	↓	↓	↓	nd	nd
Adenosine deaminase, putative	PF10_0289	↓	↓	↓	nd	nd
Spermidine synthase	PF11_0301	≈	nd	≈	nd	nd
S-adenosylmethionine synthetase, putative	PFI1090w	↓*	↓	↓	↓	nd
Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase)	PFE1050w	↓*	nd	↓	nd	nd
Lysine decarboxylase, putative	PFD0285c	↑	nd	↑	nd	nd

* = fold change between -1.5 and -2 fold
↓ = downregulated
↑ = upregulated
≈ = unchanged
nd = not determined
A large degree of overlap was observed for the response to polyamine depletion, whether PfSpdSyn, PfAdoMetDC/ODC or ODC alone were inhibited. Also, effects specific to each inhibitor could be gauged.

ISSN: 1471-2164