Validation of polymorphism assays in C. briggsae. (A) RFLP assays for snip-SNPs in three parental strains, namely AF16 (A), HK104 (H), and VT847 (V) by Hind III restriction digestion. (B) Medium indels in three parental strains showing a 100 bp deletion on fpc4171, a 145 bp insertion on fpc4140, and a 536 bp deletion on fpc0011. (C) Six additional medium indels (one for each chromosome) in AF16 and HK104 genotypes. The indels are (Chr. 1-5 and X, from left to right): cb-m142 (250 bp), cb-m21 (200 bp), cb-m205 (300 bp), cb-m172 (250 bp), cb-m103 (200 bp), and cb-m204 (250 bp). (D) Validation of small indels in AF16, HK104 and F1 heterozygotes (AH). Chr 1: bhP19 (32 bp), Chr 2: bhP21 (20 bp), Chr 3: bhP12 (39 bp), Chr 4: bhP9 (16 bp), Chr 5: bhP48 (22 bp) and Chr X: bhP24 (16 bp).