Figure 4

Mutation mapping by polymorphisms. The indels were used to map dpy(sy5001) (A) and dpy(sy5148) (B, C) and snip-SNP (cb650) was used to map lin(bh25) (D). (A) Mapping of X-linked mutation dpy(sy5001) using six medium indels (one per chromosome). W, non-mutant (phenotypically wild type) pool; M, mutant pool. (B) dpy(sy5148) localization on chromosome 2 by small indels (Chr 1: bhP19, Chr 2: bhP21, Chr 3: bhP12, Chr 4: bhP11 and Chr X: bhP26). (C) ULVs for dpy(sy5148) show linkage to bhP21. X-axis shows chromosomes whereas Y-axis linkage values. The dotted line shows the baseline for unlinked chromosomes. (D) Sac I digested PCR amplified genomic DNA of wild-type controls (A: AF16, H: HK104) and lin(bh25) mutant (M) and non-mutant (W) categories. There is a clear bias towards AF16 DNA (uncut) in the mutant pool compared to the non-mutant pool, demonstrating that lin(bh25) is linked to cb650 (chr. 1).