Figure 6

Analysis of alternative initiation of transcription from E. granulosus TGR intron 1. A. The sequence of E. granulosus TGR gene starting from intron 1 ending at exon 3. Exons 2 and 3 are shaded in grey. The putative TATA box present in intron 1 is highlighted in bold and italics. Sequences of the primers used in the PCR experiments are shown underlined and in italics: forward primers 1, 2 and 3 span intron 1 from 5' to 3'; the reverse primer derives from exon 3 sequence. B. Schematic representation of the primers used in PCR reactions, localized in the TGR sequence. C. Gel-electrophoresis of PCR reaction carried out from genomic DNA (lanes 2 to 4) and from E. granulosus cDNA (lanes 5 to 7) using a combination of the reverse primer and forward primers 1, 2 and 3 (lanes 2 and 5, lanes 3 and 6, lanes 4 and 7, respectively). Lane 1 corresponds to 100 bp plus ladder (Fermentas).