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Figure 2 | BMC Genomics

Figure 2

From: A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti

Figure 2

Relative proportion of sRNA candidates in different classes. (a) 454 sequencing: distribution of reads mapped to the S. meliloti 1021 genome and distribution of the analyzed contigs according to the general classification (Figure 3). Left circle diagram: light colored (I) and colored (II), number of reads derived from sample 1 and 2. Reads in sample 1 and 2: non-mapped, 48,159 and 57,964; rRNA genes, 67,891 and 176,848; tRNA genes, 188,121 and 79,789; repeats, 3,029 and 6,206; IGRs or ORFs, 77,326 and 140,702. Right circle diagram: light colored (I), colored (II) and dark colored (I+II) represent the number of RNA candidates derived from sample 1, sample 2, and both samples, respectively: trans-encoded sRNAs, 28, 38, 85; cis-encoded antisense sRNAs, 9, 52, 35; mRNA leader transcripts, 46, 151, 181; sense sRNAs 28, 363, 56; ORFs 0, 4, 4. (b) Illumina/Solexa sequencing: Distribution of reads mapped to the S. meliloti 1021 genome. Reads: non-mapped, 1,179,722; rRNA genes, 3,405,289; tRNA genes, 1,058,534; repeats, 111,355; IGR and ORFs, 711,851. Dark green segment: contigs for 44 putative trans-encoded sRNAs. (c) Microarray-based analysis and (d) Affymetrix Symbiosis Chip-based analysis: distribution of sRNA candidates. Segment numbers represent subtypes. Microarray data: type 1 and 2 trans-encoded sRNAs, 264 and 721 candidates; type 1, 2 and 3 cis-encoded antisense sRNAs, 25, 587 and 59; mRNA leader transcripts, 250. Affymetrix Symbiosis Chip data: type 1 and 2 trans-encoded sRNAs, 60 and 174; type 1, 2 and 3 cis-encoded antisense sRNAs, 3, 4 and 27; mRNA leader, 112.

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