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Table 1 Summary of sequencing results of the 20 sugarcane BACs using 454 Flex and the 2 BACs using Sanger method.

From: Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes

General BAC clone information

BAC sequence assembly

Sugarcane - sorghum sequence alignment

No.

BAC ID

Insert size (kb)

Chra.

GenBank ID

No. of contigs

Assembled BAC (bp)

No. of contig >10 k

No. of un-ambigious contigsb

Total (kb)

No. of ordered contigs

Total length of ordered contigs (kb)

Spanned in sugarcane (bp)

Spanned in sorghum (bp)

1

SC0126G04

75

1

FJ348725

8

86,848

4

7

85

5

79

59,458

61,142

2

SC0043O10

120

1

FJ348717

12

140,528

6

7

119

5

118

92,727

86,118

3

SC0159C20

80

2

FJ348726

22

136,575

4

19

135

8

100

57,277

41,718

4

SC0109D21

165

2

FJ348722

15

323,526

5

9

131

3

93

64,904

59,816

5

SC0215O02

40

3

FJ348732

14

90,423

4

10

79

3

38

26,478

25,458

6

SC0100B23

150

3

FJ348719

21

175,925

4

14

147

7

118

70,340

72,844

7

SC0104N03

130

4

FJ348720

15

131,592

6

13

131

6

95

57,524

50,074

8

SC0175O07

125

4

FJ348729

42

99,784

0

37

92

0

0

\

\

9

SC0019D20

0

5

 

0

0

0

0

0

0

0

\

\

10

SC0110 M04

145

5

FJ348723

15

147,646

6

11

127

4

99

41,367

37,981

11

SC0040E22

40

6

FJ348716

21

59,035

1

14

47

3

37

23,505

21,900

12

SC0204D12

90

6

FJ348731

5

96,221

3

4

96

2

76

39,070

27,519

13

SC0118L15

60

7

FJ348724

12

101,744

1

8

100

4

83

76,967

81,213

14

SC0187J16

110

7

FJ348730

11

119,506

6

10

119

4

55

31,930

27,526

15

SC0165P20

100

8

FJ348727

12

94,102

1

8

87

1

44

32,797

87,512

16

SC0150 M18

65

8

FJ348733

6

130,463

2

5

129

2

128

113,899

315,724

17

SC0108J08

60

9

FJ348721

12

113,932

3

8

104

5

98

79,663

69,498

18

SC0079A20

160

1

FJ348718

14

199,955

6

7

89

4

77

61,829

65,536

19

SC0172L01

80

10

FJ348728

19

52,373

0

18

50

0

0

\

\

20

SC0043H01

60

10

FJ348715

11

309,732

6

9

122

6

113

56,110

58,070

Total

 

1855

  

287

2,609,910

74

218

1989

72

1451

985,845

1,189,649

1

SC0118L15*

60

7

GU207345

6

62,255

3

6

62

6

62

61,625

91,428

2

SC0172L01*

80

10

GU207346

3

82,136

2

3

82

3

82

\

\

  1. *: Sanger sequence; a: corresponding sorghum chromosome; \: not available; a: chromosome number of sorghum based on the overgo probes' location.
  2. b: All 287 contigs were BLAST against each other, and those contigs with matching sequences to other contigs were removed from comparative analysis with sorghum genome