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Figure 3 | BMC Genomics

Figure 3

From: Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

Figure 3

Examples of sequencing results for cDNA clones having concatamers of TS oligo. Sequence example A reflects a cDNA clone with an insert that appears to be comprised entirely of a concatamer of 20 direct repeats of TS oligo (highlighted in alternate shades of gray) associated with a single oligo-dT reverse primer (highlighted in turquoise). The 11 intervening bases are of unknown origin. See Figure 1A for a diagrammatic representation of the process whereby this type of concatamer may arise. Sequence example B shows 9 repeats of TS oligo concatamerized to a 178 base sequence with homology to nucleotide sequences from Vitus vinifera and Arabidopsis thaliana potentially representing a plant metalloendopeptidase. The oligo-dT reverse primer is also shown. See Figure 1B for a representation of the mechanism by which this type of concatameric sequence would be generated.

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