Figure 5
From: Deep sequencing discovery of novel and conserved microRNAs in trifoliate orange (Citrus trifoliata)
Mapping of mRNA cleavage sites by RNA ligase-mediated 5' RACE. Each top strand (black) depicts a miRNA-complementary site in the target mRNA, and each bottom strand (red) depicts the miRNA. Watson-Crick pairing (vertical dashes) and G:U wobble pairing (circles) are indicated. Arrows indicate the 5' termini of mRNA fragments isolated from Citrus, as identified by cloned 5'RACE products, with the frequency of clones shown. Only cloned sequences that matched the correct gene and had 5' ends within a 100 nt window centered on the miRNA complementary site are counted. Partial mRNA sequences from target genes were aligned with miRNAs. Numbers indicate the fraction of cloned PCR products terminating at different positions. UC46-13453 was similar to AT2G38080 (NM_129364) IRX12 (IRREGULAR XYLEM 12); laccase (IRX12); UC46-24238 and UC46-17900 were similar to Populus trichocarpa (XM_002328188) cc-nbs-lrr resistance protein; UC46-20080 was similar to Populus trichocarpa (XM_002301537) cc-nbs-lrr resistance protein.