Table 2 Media and treatment for growing G. clavigera mycelium for cDNA library construction
From: Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera
Media | composition |
|---|---|
wood | 10 g/plate lodgepole pine sawdust |
starch | 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% starch |
organic nitrogen | 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% maltose |
inorganic nitrogen | 0.17% YNB, 0.1% PHP, 0.3% NaNO3, 1% maltose |
olive oil | 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% (v/v) olive oil emulsified in 0.5% tergitol (olive oil and tergitol were mixed and autoclaved separately before being added to the media) |
LPPE treatment | Cultures were grown on organic nitrogen medium for 48 h and then sprayed with LPPE |