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Figure 1 | BMC Genomics

Figure 1

From: Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation

Figure 1

Global gene expression analysis of wt and Rage-/- skin following single TPA treatment. (A) Mice of respective phenotypes were treated once with TPA, and back skin was isolated 6, 12, 24, or 48 hours after stimulation. Non-treated and acetone-treated mice served as control (0). Global gene expression analysis of RNA samples was performed on whole mouse genome oligonucleotide microarrays (n = 3 for each genotype and time point). (B) Following quantile normalization, wt genes of each kinetic from three independent animal experiments (1, 2, and 3) were ranked according to high mean and peak expression separately to filter for TPA-responsive genes. A common subset of 341 genes was identified out of the top 1000 ranked genes within each kinetic. (C) K-means clustering revealed 6 clusters of which cluster 3 and 6 shared most genes (for cluster 1, 2, 4, and 5 see Additional file 2). Black lines represent transcript levels of TPA-regulated genes in wt samples and red lines represent the corresponding genes in Rage-/- samples.

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