From: The cost of reducing starting RNA quantity for Illumina BeadArrays: A bead-level dilution experiment
Quantity of starting RNA: | 250 ng | 100 ng | 50 ng | 10 ng |
---|---|---|---|---|
Negative controls detected in UHRR | 37 | 32 | 20 | 0 |
Negative controls detected in Brain | 35 | 40 | 29 | 24 |
Negative controls detected in all array-sections | 10 | 6 | 2 | 0 |
Negative controls detected in at least one array-section | 88 | 89 | 98 | 99 |
Negative controls: median log-intensity UHRR | 5.78 | 5.79 | 5.73 | 5.58 |
Negative controls: median log-intensity Brain | 5.80 | 5.82 | 5.72 | 5.58 |
Housekeeping controls: median log-intensity UHRR | 13.46 | 13.14 | 13.09 | 11.32 |
Housekeeping controls: median log-intensity Brain | 12.47 | 12.52 | 12.03 | 10.64 |