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Table 4 Control bead-type summary

From: The cost of reducing starting RNA quantity for Illumina BeadArrays: A bead-level dilution experiment

Quantity of starting RNA:

250 ng

100 ng

50 ng

10 ng

Negative controls detected in UHRR

37

32

20

0

Negative controls detected in Brain

35

40

29

24

Negative controls detected in all array-sections

10

6

2

0

Negative controls detected in at least one array-section

88

89

98

99

Negative controls: median log-intensity UHRR

5.78

5.79

5.73

5.58

Negative controls: median log-intensity Brain

5.80

5.82

5.72

5.58

Housekeeping controls: median log-intensity UHRR

13.46

13.14

13.09

11.32

Housekeeping controls: median log-intensity Brain

12.47

12.52

12.03

10.64

  1. Giving a) the numbers of the 759 negative-control bead-types that show expression in one or more array-sections, and b) median log-intensities for negative-control and housekeeping bead-types in 100% UHRR and 100% Brain array-sections.