Figure 3

A comparison of the shared and nonshared gene content. The Venn diagram shows the shared and nonshared gene content of the isolates WB (assemblage A), GS (assemblage B) and P15 (assemblage E). The intersection displays the shared gene content between 2 or 3 genomes. Orthologs were defined using reciprocal BLAST and synteny information, as outlined in Methods. Specific genes were identified using BLASTP and TBLASTN searches with subsequent inspection of search results. Members of large gene families were not included in this comparison. The core gene content of Giardia consists of 4557 genes. Some 38 genes were found to be specific for P15, 5 genes for WB and 31 genes for GS. WB and P15 share 7 genes that are not present in GS, whereas GS and P15 share 20 genes that are not present in WB. WB and GS only share one gene which is not present in P15.