Microarray analyses of the Bombyx fat body. (A) Experimental design of microarray analyses. The dual-dye experiments were conducted between 4 M and 5 F as well as between PP and 5 F. Three biological replicates were used. (B) Hierarchical cluster analyses of three biological replicates. Green: down-regulation; red: up-regulation. (C-C") Volcano plots depicting estimated fold change (log2, x-axis) and statistical significance (-log10 P value, y-axis). Each point represents a gene, and colors correspond to the range of negative log10 P and log2 fold-change values. (D) Eight groups of differentially-expressed genes.