Table 2 Validation of microarray results by qPCR
From: Response of swine spleen to Streptococcus suis infection revealed by transcription analysis
Gene | Accession | Microarray fold change | qPCR fold change (p-value) |
|---|---|---|---|
IL1B | CK468468 | 16.7 | 258.3 (0.0117) |
S100A9 | BI402402 | 16.1 | 137.2(0.0007) |
S100A12 | CB475695 | 18.6 | 76.7 (< 0.0001) |
HSP90 | CF180819 | 3.18 | 48.5 (0.0321) |
IL8 | NM_213867 | 5.58 | 35.5 (0.0066) |
HSP70 | NM_213766 | 11.06 | 31.4 (0.0099) |
TIMP1 | NM_213857 | 4.4 | 14.6 (0.0023) |
IL6 | AF493992 | 4.8 | 10.5 (0.0074) |
SOD2 | NM_214127 | 4.6 | 10.0 (< 0.0001) |
NRAMP1 | U55068 | 3.97 | 7.2 (0.0035) |
SELE | NM_214268 | 3.5 | 5.9 (0.0002) |
PLAU | NM_213945 | 2.0 | 5.5 (0.0415) |
CCL19 | BX672579 | 2.16 | 4.2 (0.0004) |
haptocorrin | CB472702 | 3.78 | 2.1(0.0388) |
TLR-2 | NM_213761 | 2.0 | 2.1 (< 0.0001) |
ALOX15 | NM_213931 | 0.2 | 0.3 (0.038) |