Figure 4From: High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricusSemi-quantitative Reverse Transcription-PCR (RT-PCR) of candidate genes. Normalized cDNA obtained from reverse transcription of mRNA was used as template for PCR amplifications. Aliquots were taken from PCR reactions at 20, 25 and 30 cycles and analyzed by agarose gel electrophoresis.Back to article page