Table 1 Genes identified to be required for intracellular replication of L. monocytogenes within epithelial cells
From: Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling
| Â | Â | Â | fold reduction | Â | Â | Â | fold reduction | ||
|---|---|---|---|---|---|---|---|---|---|
gene name or number | protein name, or protein homology/similarity to | polar effect | intrac. | invasion/adhesion | gene name or number | protein name, or protein homology/similarity to | polar effect | intrac. | invasion/adhesion |
cell wall | Â | Â | Â | Â | protein modification | Â | Â | Â | |
1lmo0441 | penicillin-binding protein (D-alanyl-D-alanine carboxypeptidase) | no | 9.4 | 6.1 | 5lmo0618 | protein kinase | no | 11.0 | 2.0 |
2,8lmo1085 | teichoic acid biosynthesis protein B | rf | 12.3 | 2 | 1lmo0763 | hypothetical Ser/Thr protein phosphatase family protein | possible | 5.5 | 2.0/5.0 |
lmo1088 | teichoic acid biosynthesis protein B | rf | 26.2 | 1.9 | metabolism of amino acids and related molecules | Â | Â | Â | |
7lmo1713 | actin-like ATPase involved in cell morphogenesis | no | 3.6 | 3.5 | lmo0594 | homoserine O-acetyltransferase | no | 7.7 | 3.9 |
3,5,7pbpB (lmo2039) | penicillin-binding protein 2B | rf | 3.8 | 2.6 | 6,8lmo1235 | aspartokinase II a subunit | no | 5.9 | nd |
1lmo2555 | Glycosyltransferase | rf | 8.1 | 3.9/5.0 | lmo1495 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | possible | 6.2 | 1.0 |
transport/binding proteins and lipoproteins | Â | Â | Â | lmo1916 | peptidase | no | 7.0 | 2.4 | |
4lmo0135 (ctpA) | oligopeptide ABC transport system, substrate binding protein | rf | 13.6 | 2.2 | 8aroB (lmo1927) | 3-dehydroquinate synthase | rf | 2.8 | 1.4 |
4lmo0136 | oligopeptide ABC transport system, substrate binding protein | rf | 5.0 | 2.4 | 4,6ilvB (lmo1484) | acetolactate synthase (acetohydroxy-acid synthase) | rf | 5.5 | 1.8 |
lmo0137 | oligopeptide ABC transport system, permease | no | 9.7 | 1.9 | 4,6ilvC (lmo1486) | ketol-acid reductoisomerase | no | 3.2 | 3 |
4lmo0195 | ABC-type antimicrobial peptide transport system, permease | no | 6.3 | nd | lmo2051 | weakly similar to proteases | no | 4.1 | 1 |
lmo0495 | permease of the drug/metabolite transporter (DMT) superfamily | possible | 4.2 | 2.3 | lmo2694 | lysine decarboxylase | possible | 5.5 | 0 |
4lmo0584 | conserved hypothetical membrane protein, putative permease | no | 7.2 | 3.0 | lmo2770 | γ-glutamylcysteine synthetase and cyanophycin synthetase | no | 5.8 | 2.3 |
lmo0645 | amino acid transporter | no | 4.9 | 1.5 | 6serC (lmo2825) | phosphoserine aminotransferase | possible | 5.7 | 3.5 |
5lmo0650 | conserved membrane protein | possible | 2.8 | 2.0 | metabolism of nucleotides and nucleic acids | Â | Â | Â | |
lmo0787 | amino acid transporter | no | 3.4 | 1.0 | 1,5purA (lmo0055) | adenylosuccinate synthetase | no | 14.9 | /4.0 |
lmo0810 | spermidine/putrescine-binding protein | no | 7.4 | 3.1 | purQ (lmo1769) | phosphoribosylformylglycinamidine synthetase | rf | 12.0 | nd |
3lmo1003 | phosphotransferase system enzyme I | no | 3.4 | 1.0 | 8purS (lmo1771) | phosphoribosylformylglycinamidine synthetase | rf | 11.0 | 1.0 |
gbuA (lmo1014) | glycine betaine ABC transporter, ATP-binding protein | rf | 10.0 | 4.0 | pyrE (lmo1831) | orotatephosphoribosyltransferase | no | 5.5 | 2.5 |
8lmo1416 | hypothetical transporter | no | 5.3 | 1.0 | metabolism of lipids | Â | Â | Â | |
6opuCA (lmo1428) | glycine betaine/carnitine/choline ABC transporter, ATP-binding protein | rf | 15.7 | 1.7 | 5,8ispE (lmo0190) | CDP-ME synthase involved in isoprenoid biosynthesis | no | 4.7 | 2.3 |
5lmo1431 | ABC transporter, ATP binding protein | no | 4.8 | 3 | lmo1005 | 3-hydroxyisobutyrate dehydrogenase | no | 11.0 | 1.8 |
lmo1506 | ABC-type antimicrobial peptide transport system, permease | rf | 6.2 | 2.3 | lmo1363 | geranyltransferase | no | 5.0 | 2.8 |
lmo1739 | amino acid ABC transporter | rf | 5.5 | 2 | lmo2450 | carboxylesterase | possible | 4.2 | 3.0 |
3,5,7,8lmo1847 | ABC transporter specific for metal cations | no | 5.8 | 2.3 | metabolism of coenzymes and prosthetic groups | Â | Â | Â | |
lmo2124 | maltodextrin ABC transport system, permease | rf | 3.4 | 0 | 5lmo0221 | hypothetical type III pantothenate kinase | possible | 6.9 | 1.0 |
1,3oppF (lmo2192) | oligopeptide ABC-transporter, ATP-binding protein | no | 21.0 | 8.0/4.0 | 1,3pdxK (lmo0662) | pyridoxine kinase | no | 13.1 | 1/4.0 |
lmo2227 | ABC transporter, ATP-binding protein | possible | 5.8 | 1.3 | lmo1043 | molybdopterin-guanine dinucleotide biosynthesis MobB | rf | 7.9 | 1.4 |
lmo2249 | low-affinity inorganic phosphate transporter | no | 2.8 | 2.0 | lmo1932 | heptaprenyl diphosphate synthase component I | rf | 12.1 | 4.8 |
lmo2353 | hypothetical Na+/H+ antiporter | no | 4.3 | 2.3 | nadB (lmo2023) | L-aspartate oxidase | rf | 3.4 | 0 |
lmo2380 | protein involved in resistance to cholate/Na+ and in pH homeostasis | rf | 5.7 | 2.5 | 3lmo2102 | glutamine amidotransferase subunit PdxT (pyridoxine biosynthesis) | no | 4.0 | 3.3 |
lmo2430 | B. subtilis ferrichrome ABC transporter FhuG, permease | rf | 75.6 | 5.0 | lmo2566 | biotin/lipoate A/B protein ligase family | possible | 6.2 | 2.0 |
lmo2816 | transport protein | no | 6.3 | 2.0 | DNA restriction/modification and repair | Â | Â | Â | |
lmo2850 | sugar transport protein | rf | 16.5 | 0 | 5gyrB (lmo0006) | DNA gyrase subunit B | rf | 9.6 | nd |
sensors | Â | Â | Â | Â | lmo0157 | ATP-dependent helicase | possible | 2.1 | 1.0 |
lmo0799 | oxygen/light sensor with PAS domain | no | 8.3 | 2.0 | mfd (lmo0214) | transcription-repair coupling factor | possible | 8.9 | 3.7 |
lmo1508 | two-component sensor histidine kinase | no | 5.2 | 2.9 | lmo0588 | DNA photolyase | no | 9.6 | 2.0 |
membrane bioenergetics | Â | Â | Â | mutM (lmo1564) | formamidopyrimidine-DNA glycosylase | no | 5.2 | 1.1 | |
lmo0091 | ATP synthase g chain, H+-transporting two-sector ATPase | rf | 5.7 | 2.0 | lmo1751 | hypothetical RNA methyltransferase | no | 6.2 | 2.0 |
nifJ (lmo0829) | pyruvate-flavodoxin oxidoreductase | no | 6.4 | 3.6 | 8ansB (lmo1663) | asparaginyl-tRNA synthetases | no | 4.1 | 1.6 |
5atpA (lmo2531) | H+-transporting ATP synthase chain α | rf | 7.7 | 2.7 | lmo2050 | exconuclease ABC (subunit A) | no | 5.1 | 2.0 |
atpB (lmo2535) | H+-transporting ATP synthase chain β | rf | 4.4 | 5.9 | DNA recombination |  |  |  | |
mobility and chemotaxis | Â | Â | Â | 6recN (lmo1368) | RecN | no | 14.8 | 1.9 | |
lmo0680 | flagella-associated protein FlhA | rf | 7.0 | 3.1 | ruvB (lmo1532) | Holliday junction DNA helicase RuvB | possible | 9.4 | 3.9 |
6lmo0700 | flagellar motor switch protein FliY | rf | 5.8 | 1.7 | 8recS (lmo1942) | similar to ATP-dependent DNA helicase | no | 7.0 | 2.3 |
cell surface proteins | Â | Â | Â | regulation | Â | Â | Â | Â | |
1,2,6vip (lmo0320) | putative peptidoglycan bound protein with LPXTG motif | no | 6.4 | 4.5 | 3agrA (lmo0051) | 2-component response regulator protein | no | 3.4 | 1.0 |
8lmo0327 | protein with LPXTG motif, putative murein hydrolase activity | no | 6.9 | 3.0 | 6lmo0294 | transcription regulator, LysR-gltR family | no | 7.4 | 3.1 |
4lmo0514 | internalin-like protein with LPXTG motif | no | 6.4 | 1.4 | lmo0535 | transcription regulator, LacI family | no | 9.5 | 1.0 |
4lmo0576 | hypothetical cell wall associated protein | no | 4.2 | 1.5 | 3fur (lmo1956) | transcriptional regulator, Fur family | no | 5.5 | 1.0 |
2,8lmo1666 | peptidoglycan linked protein with LPXTG motif | no | 6.8 | 1.8 | lmo1994 | transcription regulators, LacI family | no | 9.0 | 1.4 |
lmo2026 | hypothetical peptidoglycan bound protein with LPXTG motif | no | 8.7 | 1.4 | RNA modification | Â | Â | Â | Â |
5lmo2504 | cell wall binding protein, peptidase-related enzyme | no | 4.8 | 2.0 | lmo0241 | hypothetical RNA methyltransferase, trmH family protein | rf | 4.1 | 1.0 |
1ami (lmo2558) | autolysin, N-acetylmuramoyl-L-alanine amidase | no | 3.8 | 5.3 | 1,3,5lmo1434 | RNA-metabolising metallo-β-lactamase | no | 9.6 | 3/22 |
metabolism of carbohydrates and related molecules | Â | Â | Â | miscellaneous | Â | Â | Â | Â | |
glpQ (lmo0052) | transmembrane protein with phosphoesterase domain | possible | 4.1 | 2.0 | lmo0066 | toxin component of A/B toxin | rf | 2.8 | 1.0 |
8lmo0182 | α-xylosidase and α-glucosidase | rf | 7.9 | 1.3 | 4lmo0585 | secreted protein | no | 4.8 | 1.0 |
lmo0261 | phospho-β-glucosidase | no | 3.4 | 0 | lmo0587 | secreted protein, YapH from Y. pestis, cell wall surface protein | no | 3.4 | 1.0 |
8lmo0271 | phospho-β-glucosidase | possible | 4.1 | 1.0 | unknown proteins |  |  |  |  |
lmo0517 | phosphoglycerate mutase | no | 13.8 | 2.3 | lmo0276 | hypothetical hydrolase, HAD superfamily | possible | 5.2 | 1.3 |
2lmo1031 | hypothetical L-fucose isomerase | rf | 11.1 | 2.1 | 2lmo0313 | hypothetical hydrolase, PHP superfamily | no | 2.8 | 1.0 |
2lmo1032 | Transketolase | rf | 14.4 | Â | 4lmo0590 | hypothetical DAK2/DegV domain-containing protein | possible | 9.6 | 1.0 |
lmo1166 | NADPH-dependent butanol dehydrogenaseI | possible | 4.2 | 1.0 | lmo0765 | unknown protein | possible | 10.4 | 3.0 |
4glpD (lmo1293) | glycerol-3-phosphate dehydrogenase | no | 4.8 | 1.0 | 4,6,7lmo0794 | B. subtilis YwnB protein | no | 12.4 | 2.0 |
6lmo1244 | weakly similar to phosphoglycerate mutase 1 | no | 8.3 | 2.0 | lmo1379 | B. subtilis SpoIIIJ protein, hypothetical membrane protein | no | 4.8 | 3.0 |
lmo2005 | Oxidoreductase | no | 7.4 | 3.9 | 3lmo1402 | B. subtilis YmcA protein | no | 2.1 | 2.0 |
lmo2015 | α-mannosidase | no | 6.4 | 1.7 | lmo1575 | phosphoesterase, DHH superfamily | no | n.d. | 1.0 |
lmo2134 | fructose-1,6-biphosphate aldolase type II | no | 5.2 | 2.4 | 6,8lmo1700 | unknown protein | no | 4.6 | 1.3 |
lmo2172 | propionate CoA-transferase | possible | 5.3 | 1.3 | 4lmo1830 | short chain dehydrogenase | no | 11.7 | 2.0 |
lmo2247 | Oxidoreductase | possible | 5.0 | 2.4 | lmo1866 | hypothetical phosphotransferase | possible | 7.1 | 2.2 |
8lmo2446 | Glycosidase | no | 9.9 | 2.5 | lmo1920 | unknown protein | Â | 16.5 | 1.0 |
lmo2586 | formate dehydrogenase α-chain | possible | 2.4 | 2.2 | lmo2516 | conserved hypothetical protein | possible | 3.6 | 1.2 |
6lmo2660 | Transketolase | rf | 3.0 | 2.6 | lmo2639 | unknown protein, contains DUF1312 domain | possible | 5.5 | 1.0 |
lmo2764 | xylose operon regulatory protein and to glucose kinase | rf | 3.6 | 2.1 | no similarity | Â | Â | Â | Â |
2lmo2781 | β-glucosidase | rf | 6.5 | 2.0 | lmo0729 | no similarity | no | 4.8 | 1.0 |
lmo2831 | Phosphoglucomutase | no | 5.3 | 2.8 | 2lmo1188 | no similarity | no | 5.1 | 3.5 |
| Â | Â | Â | Â | Â | lmo1219 | no similarity | no | 4.8 | 2.0 |
| Â | Â | Â | Â | Â | lmo2129 | no similarity | no | 5.8 | 1.6 |