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Table 1 Primer locations in the exons or flanking untranslated regions1

From: An Sp185/333 gene cluster from the purple sea urchin and putative microsatellite-mediated gene diversification

Primer2

Sequence

Strand3

Notes

5' UTR.1

YTDTAGCATCGGAGAKACCT4

S

5' untranslated region of all genes

F2

AAGMGATTWCAATGAACKRCGAG

S

In the second exon ~500 bp from 5' end of all genes

F5

GGAACYGARGAMGGATCTC

S

In the second exon ~1.4 kb from the 5' end of most genes

F6

GAAGAAGAAACTGATGCTGCC

S

In the second exon ~900 bp from the start codon in all genes.

LR1

ATCRTYGCCATYSTGGCYG

AS

In the first exon ~50 bp from the start codon in all genes.

R5

AAATGGCGGTCCGATGRGTG

AS

In the second exon ~800 bp from the start codon in most genes

R6

GAGAMGAAGAAACTGATGCTGC

AS

In the second exon ~900 bp from the start codon in all genes.

R9

CGACATYTTCACCACYTDAAG

AS

In the second exon ~ 1.5 kb from the 5' end of most genes

3' UTR.1

GTCGCYGAGGTGTAGAATTW

AS

3' end of all genes

3' UTF-1

CGTCATAACCGTACCAAAGAC

S

3' end of some genes

  1. 1see also [15, 19]
  2. 2F, forward; R, reverse
  3. 3S = sense; AS = antisense
  4. 4D = A, G, or T; K = G or T; Y = C or T; R = A or G; M = A or C