Figure 1

Changes in specific growth rate during evolution experiments. The time course of specific growth rates in the evolution experiments (a) without ethanol and (b) with 5% (v/v) ethanol are plotted. In the case with ethanol stress, 6 parallel series of experiments were performed starting from strain P obtained at 912 hours in the experiment shown in (a). The cells obtained after 2,500 hours of cultivation under ethanol stress were named "strain A"-"strain F," in descending order of the final growth rate.