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Table 2 Percentage of polymorphic markers for successfully amplified primer pairs

From: Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

Motifs Primers designed Amplified primersa (%) Polymorphic primersb (%)
Di-nucleotides 666 591(88.74) 339(50.90)
AG 338 306(90.53) 193(57.10)
AT 294 254(86.39) 128(43.54)
AC 34 31(91.18) 18(52.94)
Tri-nucleotides 164 150(91.46) 92(58.10)
AAG 61 56(91.80) 37(60.66)
ATG 35 33(94.29) 20(57.14)
AGG 18 17(94.44) 8(44.44)
AAT 21 19(90.48) 11(52.38)
AAC 16 14(87.50) 8(50.00)
GC-rich TNRs 13 11(84.62) 8(61.54)
Tetra-nucleotides 30 29(96.67) 16(53.33)
Penta-nucleotides 30 24(80.00) 13(43.33)
Total 890 794(89.21) 460(51.69)
  1. a Percentage of successfully amplified SSRs per designed primer pairs
  2. b Percentage of polymorphic markers per successfully amplified primer pairs based on the screening of six B. napus lines.